Ohishi K, Suzuki H, Maruyama T, Yamamoto T, Funahashi S, Miki K, Ikawa Y, Sugimoto M
Corporate Research and Development Laboratory, Tonen Corp, Saitama, Japan.
Am J Vet Res. 1990 Aug;51(8):1170-3.
Three kinds of recombinant vaccinia virus (RVV)--mO-HA/ATI, LO1-HA/ATI and mO-HA/7.5kD--expressing bovine leukosis virus (BLV) envelope glycoprotein (gp60) were constructed. The BLV envelope gene of RVV mO-HA/ATI and LO1-HA/ATI or of RVV mO-HA/7.5kD was expressed under control of the promoter of A-type inclusion body (ATI) protein gene of cow-pox virus or vaccinia virus 7.5-kD protein gene, respectively. The vaccinia virus strain, LC16mO, was used as vector for RVV mO-HA/ATI and mO-HA/7.5kD, and strain LO-1 was used for RVV LO1-HA/ATI. Strains LC16mO and LO-1 are attenuated vaccine virus strains originating from the Lister original vaccinia virus. All 3 kinds of constructed RVV expressed gp60 in cultured rabbit kidney cells after infection; mO-HA/ATI expressed more antigen than did mO-HA/7.5kD. Rabbits vaccinated with RVV produced considerable antibody capable of inhibiting syncytium formation, as well as antibody with virion-binding ability. The RVV that used ATI promoter induced higher antibody titer than did the RVV that used 7.5-kD promoter. Results indicate that BLV gp60 is responsible for induction of neutralizing antibodies that suppress in vitro formation of syncytia among BLV-infected cells. Applicability of RVV, especially those using ATI promoter, was evaluated in a vaccine against bovine leukosis.
构建了三种表达牛白血病病毒（BLV）包膜糖蛋白（gp60）的重组痘苗病毒（RVV）——mO-HA/ATI、LO1-HA/ATI和mO-HA/7.5kD。RVV mO-HA/ATI和LO1-HA/ATI的BLV包膜基因或RVV mO-HA/7.5kD的BLV包膜基因分别在牛痘病毒A型包涵体（ATI）蛋白基因启动子或痘苗病毒7.5-kD蛋白基因启动子的控制下表达。痘苗病毒株LC16mO用作RVV mO-HA/ATI和mO-HA/7.5kD的载体，LO-1株用于RVV LO1-HA/ATI。LC16mO和LO-1株是源自李斯特原始痘苗病毒的减毒疫苗病毒株。所有三种构建的RVV在感染后在培养的兔肾细胞中均表达gp60；mO-HA/ATI表达的抗原比mO-HA/7.5kD更多。用RVV免疫的兔子产生了相当数量的能够抑制合胞体形成的抗体以及具有病毒体结合能力的抗体。使用ATI启动子的RVV诱导的抗体滴度高于使用7.5-kD启动子的RVV。结果表明，BLV gp60负责诱导中和抗体，该抗体可抑制BLV感染细胞之间的体外合胞体形成。在牛白血病疫苗中评估了RVV的适用性，尤其是那些使用ATI启动子的RVV。
