Ohishi K, Maruyama T, Shida H, Nishimaki J, Miki K, Sagata N, Ikawa Y, Sugimoto M
Corporate Research and Development Laboratory, Toa Nenryo Kogyo K.K., Saitama, Japan.
Vaccine. 1988 Oct;6(5):428-32. doi: 10.1016/0264-410x(88)90144-2.
We constructed a recombinant vaccinia virus (RVV) expressing envelope (env) glycoprotein (gp51) of bovine leukaemia virus (BLV): the expression of gp51 was detected by Western blot of the lysates of rabbit kidney cells infected with the RVV. The rabbits inoculated intradermally with the RVV alone failed to induce detectable anti-gp51 antibodies even 10 weeks after immunization. However, when these animals were boosted with inactivated BLV virion in saline, significant levels of anti-gp51 antibodies were induced as shown both in Western blot and immunodiffusion analyses. In these animals, antibodies against gag product (p24) were not detected. On the other hand, the rabbits inoculated with wild-type vaccinia virus and boosted similarly three times with the BLV virion in saline did not induce detectable anti-gp51 antibodies at all. The present experiment revealed that the RVV possessed the capability to endow immunological memory without inducing apparent anti-gp51 antibody responses, meaning that the RVV activated helper T cells far more strongly than B cells. The applicability of the RVV to vaccine development is discussed.
我们构建了一种表达牛白血病病毒（BLV）包膜（env）糖蛋白（gp51）的重组痘苗病毒（RVV）：通过对感染RVV的兔肾细胞裂解物进行蛋白质印迹法检测到了gp51的表达。仅皮内接种RVV的兔子即使在免疫10周后也未能诱导出可检测到的抗gp51抗体。然而，当用盐水中的灭活BLV病毒粒子对这些动物进行加强免疫时，蛋白质印迹法和免疫扩散分析均显示诱导出了显著水平的抗gp51抗体。在这些动物中，未检测到针对gag产物（p24）的抗体。另一方面，接种野生型痘苗病毒并同样用盐水中的BLV病毒粒子进行三次加强免疫的兔子根本未诱导出可检测到的抗gp51抗体。本实验表明，RVV具有赋予免疫记忆的能力，而不诱导明显的抗gp51抗体反应，这意味着RVV激活辅助性T细胞的能力远强于B细胞。本文还讨论了RVV在疫苗开发中的适用性。
