Ohishi K, Suzuki H, Yamamoto T, Maruyama T, Miki K, Ikawa Y, Numakunai S, Okada K, Ohshima K, Sugimoto M
Corporate Research and Development Laboratory, Tonen Corporation, Saitama, Japan.
J Gen Virol. 1991 Aug;72 ( Pt 8):1887-92. doi: 10.1099/0022-1317-72-8-1887.
The effects of vaccination of sheep with a recombinant vaccinia virus (rVV) expressing the bovine leukaemia virus (BLV) envelope glycoprotein (gp60) were studied by determining BLV titres in peripheral blood leukocytes after vaccination and challenge. The proliferation of BLV was suppressed markedly, not only when rVV was inoculated prior to challenge with BLV, but also when it was inoculated after challenge. These results indicate that vaccination with rVV induces protective immunity that can suppress the growth of BLV in carrier animals. Since rVV induced a strong anti-BLV delayed-type hypersensitivity response without producing detectable levels of binding or neutralizing antibodies, and there was no apparent correlation between the humoral immune response and BLV proliferation, a cell-mediated immune response was assumed to play a major role in protective immunity.
通过测定接种和攻毒后外周血白细胞中的牛白血病病毒（BLV）滴度，研究了用表达牛白血病病毒包膜糖蛋白（gp60）的重组痘苗病毒（rVV）对绵羊进行疫苗接种的效果。不仅在接种BLV之前接种rVV时，而且在接种BLV之后接种rVV时，BLV的增殖均受到明显抑制。这些结果表明，用rVV进行疫苗接种可诱导保护性免疫，从而抑制携带动物体内BLV的生长。由于rVV诱导了强烈的抗BLV迟发型超敏反应，而未产生可检测水平的结合抗体或中和抗体，并且体液免疫反应与BLV增殖之间没有明显相关性，因此推测细胞介导的免疫反应在保护性免疫中起主要作用。
