利用阿维链霉菌pteB对2-辛烯酰辅酶A羧化酶/还原酶的表征
Characterization of 2-octenoyl-CoA carboxylase/reductase utilizing pteB from Streptomyce avermitilis.
作者信息
Yoo Hye-Gyeong, Kwon So-Yeon, Kim Suji, Karki Suman, Park Zee-Yong, Kwon Hyung-Jin
机构信息
Department of Biological Science, Division of Bioscience and Bioinformatics, Myongji University, Yongin, Republic of Korea.
出版信息
Biosci Biotechnol Biochem. 2011;75(6):1191-3. doi: 10.1271/bbb.110003. Epub 2011 Jun 13.
The filipin biosynthetic gene cluster of Streptomyces avermitilis contains pteB, a homolog of crotonyl-CoA carboxylase/reductase. PteB was predicted to be 2-octenoyl-CoA carboxylase/reductase, supplying hexylmalonyl-CoA to filipin biosynthesis. Recombinant PteB displayed selective reductase activity toward 2-octenoyl-CoA while generating a broad range of alkylmalonyl-CoAs in the presence of bicarbonate.
阿维链霉菌的菲律宾菌素生物合成基因簇包含pteB，它是巴豆酰辅酶A羧化酶/还原酶的同源物。预测PteB为2-辛烯酰辅酶A羧化酶/还原酶，为菲律宾菌素生物合成提供己基丙二酰辅酶A。重组PteB对2-辛烯酰辅酶A表现出选择性还原酶活性，同时在存在碳酸氢盐的情况下生成多种烷基丙二酰辅酶A。
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