Department of Oral Diagnosis, Microbiology and Immunology Division, Piracicaba Dental School, University of Campinas (UNICAMP), Piracicaba, Brazil.
Med Mycol. 2012 Jan;50(1):43-9. doi: 10.3109/13693786.2011.586133. Epub 2011 Jun 15.
The objectives of this study were to evaluate clinical isolates of Candida albicans, particularly their adhesion to and invasion of gingival human fibroblasts in culture and to measure nitric oxide concentration (NO) produced by fibroblasts in the presence of these yeasts. Sixteen strains of C. albicans isolated from patients with chronic periodontitis and diabetes mellitus type II were divided on the basis of phenotypic tests into two groups, i.e., highly or weakly hydrophobic. Primary cultures of human fibroblasts were isolated from gingival biopsies and after subsequent subcultures, the cells were seeded into culture plates and incubated for 24 h. C. albicans strains were inoculated into these plates and maintained for 2 and 4 h to assess their adhesion and invasion, respectively. The number of adherent or invasive yeasts was evaluated by assessing colony-forming units (CFU). The production of NO by fibroblasts was also quantified. The results showed that strains with high hydrophobicity had a greater ability to adhere and invade fibroblasts (p < 0.05, ANOVA and Tukey). The production of NO was higher for the most hydrophobic strains, but did not reach statistical difference with the weakly hydrophobic isolates. These data indicated that the hydrophobicity may play a role in the adhesion and invasion of C. albicans in fibroblast cultures.
本研究的目的在于评估白色念珠菌的临床分离株，特别是其在体外对培养的人牙龈成纤维细胞的黏附与侵袭能力，并测定这些酵母菌存在时成纤维细胞产生的一氧化氮（NO）浓度。从慢性牙周炎和 2 型糖尿病患者中分离出 16 株白色念珠菌，根据表型试验将其分为高度或低度疏水性两组。从牙龈活检中分离出原代人成纤维细胞，经后续传代培养后，将细胞接种于培养板中孵育 24 小时。将白色念珠菌菌株接种于这些平板中，分别孵育 2 小时和 4 小时，以评估其黏附与侵袭能力。通过评估集落形成单位（CFU）来评估黏附或侵袭的酵母菌数量。还定量测定了成纤维细胞产生的 NO。结果表明，高疏水性菌株具有更强的黏附与侵袭成纤维细胞的能力（p < 0.05，ANOVA 和 Tukey）。最疏水性菌株产生的 NO 更高，但与低度疏水性分离株相比，差异无统计学意义。这些数据表明，疏水性可能在白色念珠菌在成纤维细胞培养物中的黏附和侵袭中发挥作用。
