Urzúa B, Hermosilla G, Gamonal J, Morales-Bozo I, Canals M, Barahona S, Cóccola C, Cifuentes V
Departamento de Ciencias Fisicas y Quimicas.
Med Mycol. 2008 Dec;46(8):783-93. doi: 10.1080/13693780802060899.
The term periodontitis encompasses several polymicrobial infectious diseases, of multifactorial etiology, with chronic and aggressive forms. In spite of the etiopathogenic differences between these two forms of the disease, few studies have analyzed the subgingival colonization by yeast. The objective of this investigation was to analyze the composition of the yeast microbiota present in the mucosa and subgingival sites of healthy individuals and patients with aggressive and chronic periodontitis. For this, samples were recovered from these two locations and the yeast recovered identified by phenotypic and genotypic methods. Patients with chronic periodontitis showed significant differences in relation to the other groups with respect to carrier status (69.2% versus 35.7% of healthy individuals; [chi(i)(2) test; p=0.014]), the total number of isolated colony forming units or CFU (mean and ranges 281.6 (0-6048) [K-W(2)=6.998; p=0.03]), the Simpson diversity index (I) in site b (I(b)=0.344 versus healthy subjet and aggresive periodontitis where I=0 [multiple t-test comparisons with the Bonferronni correction, p<0.05]), and the species profile. Interestingly, in spite of the varied profiles of the species present in the mucosa of the three groups analyzed we noted that only C. albicans and C. dubliniensis were capable of colonizing the periodontal pockets in patients with chronic periodontitis, while only C. albicans was identified in the subgingiva of healthy individuals and patients with aggressive periodontitis.
术语“牙周炎”涵盖多种多微生物感染性疾病，其病因多因素，有慢性和侵袭性两种形式。尽管这两种疾病形式在病因学上存在差异，但很少有研究分析酵母在龈下的定植情况。本研究的目的是分析健康个体、侵袭性牙周炎患者和慢性牙周炎患者黏膜及龈下部位酵母微生物群的组成。为此，从这两个部位采集样本，并通过表型和基因型方法对分离出的酵母进行鉴定。慢性牙周炎患者在携带状态方面与其他组存在显著差异（69.2% 对比健康个体的35.7%；[卡方检验；p = 0.014]），分离出的菌落形成单位总数或CFU（均值及范围281.6(0 - 6048)[K - W(2)=6.998；p = 0.03]），位点b的辛普森多样性指数（I）（I(b)=0.344，对比健康受试者和侵袭性牙周炎患者，其I = 0[采用Bonferronni校正的多重t检验比较，p < 0.05]）以及菌种分布。有趣的是，尽管分析的三组黏膜中存在的菌种分布各异，但我们注意到，只有白色念珠菌和都柏林念珠菌能够在慢性牙周炎患者的牙周袋中定植，而在健康个体和侵袭性牙周炎患者的龈下仅鉴定出白色念珠菌。
