Gomes Cinthya Cristina, Guimarães Ludmila Silva, Pinto Larissa Christina Costa, Camargo Gabriela Alessandra da Cruz Galhardo, Valente Maria Isabel Bastos, Sarquis Maria Inêz de Moura
Universidade Federal Fluminense, Faculdade de Odontologia, Departamento de Formação Específica, Nova Friburgo, RJ, Brasil.
Universidade Federal Fluminense, Faculdade de Odontologia, Programa de Pós-graduação em Odontologia, Nova Friburgo, RJ, Brasil.
J Appl Oral Sci. 2017 May-Jun;25(3):274-281. doi: 10.1590/1678-7757-2016-0432.
The aim of this study was to investigate the prevalence of isolated Candida albicans from periodontal endodontic lesions in diabetic and normoglycemic patients, and the fungi's virulence in different atmospheric conditions.
A case-control study was conducted on 15 patients with type 2 diabetes mellitus (G1) and 15 non-diabetics (G2) with periodontal endodontic lesions. Samples of root canals and periodontal pockets were plated on CHROMagar for later identification by polymerase chain reaction (PCR) and virulence test.
C. albicans was identified in 79.2% and 20.8% of the 60 samples collected from diabetic and normoglycemic patients, respectively. Of the 30 samples collected from periodontal pockets, 13 showed a positive culture for C. albicans, with 77% belonging to G1 and 23% to G2. Of the 11 positive samples from root canals, 82% were from G1 and 18% from G2. Production of proteinase presented a precipitation zone Pz<0.63 of 100% in G1 and 72% in G2, in redox and negative (Pz=1), under anaerobic conditions in both groups. Hydrophobicity of the strains from G1 indicated 16.4% with low, 19.3% with moderate, and 64.3% with high hydrophobicity in redox. In G2, 42.2% had low, 39.8% had moderate, 18% had high hydrophobicity in redox. In anaerobic conditions, G1 showed 15.2% with low, 12.8% with moderate, and 72% with high hydrophobicity; in G2, 33.6% had low, 28.8% had moderate, and 37.6% had high hydrophobicity. There was statistical difference in the number of positive cultures between G1 and G2 (p<0.05) with predominance in G1. There was statistical difference for all virulence factors, except hemolysis (p=0.001).
Candida albicans was isolated more frequently and had higher virulence in diabetic patients.
本研究旨在调查糖尿病患者和血糖正常患者牙周牙髓病变中白色念珠菌的分离率，以及该真菌在不同大气条件下的毒力。
对15例患有2型糖尿病的患者（G1组）和15例患有牙周牙髓病变的非糖尿病患者（G2组）进行病例对照研究。将根管和牙周袋样本接种于科玛嘉显色培养基上，随后通过聚合酶链反应（PCR）和毒力试验进行鉴定。
从糖尿病患者和血糖正常患者收集的60份样本中，白色念珠菌的鉴定率分别为79.2%和20.8%。在从牙周袋收集的30份样本中，13份白色念珠菌培养呈阳性，其中77%属于G1组，23%属于G2组。在从根管收集的11份阳性样本中，82%来自G1组，18%来自G2组。在氧化还原和阴性（Pz = 1）条件下，两组在厌氧条件下，G1组蛋白酶产生呈现沉淀区Pz<0.63的比例为100%，G2组为72%。G1组菌株在氧化还原条件下的疏水性表明，低疏水性占16.4%，中等疏水性占19.3%，高疏水性占64.3%。在G2组中，氧化还原条件下42.2%为低疏水性，39.8%为中等疏水性，18%为高疏水性。在厌氧条件下，G1组低疏水性占15.2%，中等疏水性占12.8%，高疏水性占72%；在G2组中，33.6%为低疏水性，28.8%为中等疏水性，37.6%为高疏水性。G1组和G2组之间阳性培养物数量存在统计学差异（p<0.05），G1组占优势。除溶血外，所有毒力因子均存在统计学差异（p = 0.001）。
白色念珠菌在糖尿病患者中分离频率更高且毒力更强。
