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A protein with sequence identity to Skp (FirA) supports protein translocation into plasma membrane vesicles of Escherichia coli.

作者信息

Thome B M, Hoffschulte H K, Schiltz E, Müller M

机构信息

Institute of Biochemistry, University of Freiburg, FRG.

出版信息

FEBS Lett. 1990 Aug 20;269(1):113-6. doi: 10.1016/0014-5793(90)81132-8.

DOI:10.1016/0014-5793(90)81132-8
PMID:2167239
Abstract

We have purified to homogeneity a 15 kDa-protein from a ribosomal salt extract of Escherichia coli that compensates in vitro a defect of SecA but not of SecB. Removal of this protein from a cell-free transcription/translation system impairs translocation into plasma membrane vesicles of the precursors of LamB and to a lesser degree also of OmpA. These results suggest a role of the 15 kDa-protein in bacterial protein export. The NH2-terminal 35 amino acids were found to be identical to those of the skp (firA) gene product, to which several putative functions have previously been attributed.

摘要

相似文献

1
A protein with sequence identity to Skp (FirA) supports protein translocation into plasma membrane vesicles of Escherichia coli.
FEBS Lett. 1990 Aug 20;269(1):113-6. doi: 10.1016/0014-5793(90)81132-8.
2
Cytosolic factor purified from Escherichia coli is necessary and sufficient for the export of a preprotein and is a homotetramer of SecB.从大肠杆菌中纯化的胞质因子对于前体蛋白的输出是必需且充分的，并且是SecB的同四聚体。
Proc Natl Acad Sci U S A. 1989 Apr;86(8):2728-32. doi: 10.1073/pnas.86.8.2728.
3
Purification of the Escherichia coli secB gene product and demonstration of its activity in an in vitro protein translocation system.大肠杆菌secB基因产物的纯化及其在体外蛋白质转运系统中的活性证明。
J Biol Chem. 1989 Feb 5;264(4):2242-9.
4
Comparative characterization of SecA from the alpha-subclass purple bacterium Rhodobacter capsulatus and Escherichia coli reveals differences in membrane and precursor specificity.来自α-亚类紫色细菌荚膜红细菌和大肠杆菌的SecA的比较表征揭示了膜和前体特异性的差异。
J Bacteriol. 1997 Jun;179(12):4003-12. doi: 10.1128/jb.179.12.4003-4012.1997.
5
A mutation of Escherichia coli SecA protein that partially compensates for the absence of SecB.一种大肠杆菌SecA蛋白的突变，可部分补偿SecB缺失的影响。
J Bacteriol. 1993 Apr;175(8):2255-62. doi: 10.1128/jb.175.8.2255-2262.1993.
6
In vivo and in vitro characterization of the secA gene product of Bacillus subtilis.枯草芽孢杆菌secA基因产物的体内和体外特性分析
J Bacteriol. 1992 Jul;174(13):4308-16. doi: 10.1128/jb.174.13.4308-4316.1992.
7
A truncated Bacillus subtilis SecA protein consisting of the N-terminal 234 amino acid residues forms a complex with Escherichia coli SecA51(ts) protein and complements the protein translocation defect of the secA51 mutant.由N端234个氨基酸残基组成的截短型枯草芽孢杆菌SecA蛋白与大肠杆菌SecA51（ts）蛋白形成复合物，并弥补了secA51突变体的蛋白质转运缺陷。
J Biochem. 1994 Dec;116(6):1287-94. doi: 10.1093/oxfordjournals.jbchem.a124677.
8
Both an N-terminal 65-kDa domain and a C-terminal 30-kDa domain of SecA cycle into the membrane at SecYEG during translocation.在转运过程中，SecA的N端65 kDa结构域和C端30 kDa结构域都会在SecYEG处循环进入膜内。
Proc Natl Acad Sci U S A. 1997 May 27;94(11):5574-81. doi: 10.1073/pnas.94.11.5574.
9
The molecular chaperone SecB is released from the carboxy-terminus of SecA during initiation of precursor protein translocation.在前体蛋白转运起始过程中，分子伴侣SecB从SecA的羧基末端释放出来。
EMBO J. 1997 Oct 15;16(20):6105-13. doi: 10.1093/emboj/16.20.6105.
10
Site-specific antibodies against the PrlA (secY) protein of Escherichia coli inhibit protein export by interfering with plasma membrane binding of preproteins.针对大肠杆菌PrlA（secY）蛋白的位点特异性抗体通过干扰前体蛋白与质膜的结合来抑制蛋白质输出。
Proc Natl Acad Sci U S A. 1989 Mar;86(6):1895-9. doi: 10.1073/pnas.86.6.1895.

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Characterization of the gene encoding a 26-kilodalton protein (OMP26) from nontypeable Haemophilus influenzae and immune responses to the recombinant protein.不可分型流感嗜血杆菌编码26千道尔顿蛋白（OMP26）的基因特征及对重组蛋白的免疫反应。
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10
Identification and sequence analysis of the gene mutated in the conditionally lethal outer membrane permeability mutant SS-C of Salmonella typhimurium.
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