HIV-1 准种在肠道和外周血隔室之间缺乏分隔。
Lack of compartmentalization of HIV-1 quasispecies between the gut and peripheral blood compartments.
机构信息
Clinical and Molecular Retrovirology Section, Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.
出版信息
J Infect Dis. 2011 Jul 15;204(2):309-14. doi: 10.1093/infdis/jir259.
Abstract
Compartmental differences in human immunodeficiency virus type 1 (HIV-1) between the gut and peripheral blood and within the gut were examined. Biopsy specimens from the colon and ileum and peripheral blood samples were collected from chronically HIV-1-infected individuals. HIV-1 envelope sequences were examined from cell-associated DNA and RNA and virion RNA. Phylogenetic analysis revealed no evidence of compartmentalization of HIV-1 between the gut and peripheral blood and within the gut (colon and ileum). HIV-1 sequences detected in the gut were transcriptionally active and were also found in peripheral blood from matching time points, providing evidence of ongoing virus production in the gut and equilibrium of HIV-1 between the gut and peripheral blood compartments.
摘要
本研究旨在探讨人类免疫缺陷病毒 1 型（HIV-1）在肠道和外周血之间以及肠道内的区室差异。采集慢性 HIV-1 感染者的结肠和回肠活检标本及外周血样本。从细胞相关 DNA 和 RNA 以及病毒 RNA 中检测 HIV-1 包膜序列。系统进化分析未发现 HIV-1 在肠道和外周血之间以及肠道内（结肠和回肠）存在区室化。在肠道中检测到的 HIV-1 序列具有转录活性，并且在匹配的时间点也存在于外周血中，这为病毒在肠道持续复制以及肠道和外周血之间 HIV-1 平衡提供了证据。
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