用于诊断人冠状病毒229E感染的核酸探针的特性分析。
Characterization of a nucleic acid probe for the diagnosis of human coronavirus 229E infections.
作者信息
Myint S, Harmsen D, Raabe T, Siddell S G
机构信息
MRC Common Cold Unit, Salisbury, England.
出版信息
J Med Virol. 1990 Jun;31(2):165-72. doi: 10.1002/jmv.1890310216.
Abstract
A cDNA copy of the HCV229E nucleocapsid protein gene was isolated and characterized. Sequence analysis predicts a nucleocapsid polypeptide of 389 amino acids with a molecular weight (mol. wt.) of 43,450. Single strand RNA probes derived from the cDNA copy hybridize specifically to HCV229E RNA and approximately 50 pg of intracellular viral RNA can be readily detected. The application of nucleic acid hybridization as a routine procedure for the diagnosis of HCV229E infection is discussed.
摘要
分离并鉴定了丙型肝炎病毒229E（HCV229E）核衣壳蛋白基因的互补DNA（cDNA）拷贝。序列分析预测其核衣壳多肽由389个氨基酸组成，分子量为43450。源自该cDNA拷贝的单链RNA探针可与HCV229E RNA特异性杂交，并且大约50皮克的细胞内病毒RNA能够轻易被检测到。文中讨论了将核酸杂交作为诊断HCV229E感染的常规方法的应用。
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