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重组转化生长因子β3：分离骨细胞中的生物学活性及受体结合特性

Recombinant transforming growth factor type beta 3: biological activities and receptor-binding properties in isolated bone cells.

作者信息

ten Dijke P, Iwata K K, Goddard C, Pieler C, Canalis E, McCarthy T L, Centrella M

机构信息

Oncogene Science Inc., Manhasset, New York 11030.

出版信息

Mol Cell Biol. 1990 Sep;10(9):4473-9. doi: 10.1128/mcb.10.9.4473-4479.1990.

DOI:10.1128/mcb.10.9.4473-4479.1990
PMID:2167432
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC361033/
Abstract

We have recently cloned the cDNA for transforming growth factor type beta 3 (TGF-beta 3), a new member of the TGF-beta gene family. We examined the biological effects of recombinant TGF-beta 3 protein in osteoblast-enriched bone cell cultures. In this report we demonstrate that TGF-beta 3 is a potent regulator of functions associated with bone formation, i.e., mitogenesis, collagen synthesis, and alkaline phosphatase activity. In a direct comparison between TGF-beta 3 and TGF-beta 1, TGF-beta 3 appeared to be three- to fivefold more potent than TGF-beta 1. Our cross-linking experiments with iodinated TGF-beta showed that in osteoblast-enriched bone cell cultures, both TGF-beta 3 and TGF-beta 1 associated with the same three cell surface binding sites. Scatchard analysis of receptor competition studies indicated the presence of high-affinity binding sites for TGF-beta 3 in the picomolar range. TGF-beta 3 showed an approximately fourfold-higher apparent affinity than TGF-beta 1 in overall binding.

摘要

我们最近克隆了转化生长因子β3（TGF-β3）的cDNA，它是TGF-β基因家族的一个新成员。我们在富含成骨细胞的骨细胞培养物中检测了重组TGF-β3蛋白的生物学效应。在本报告中，我们证明TGF-β3是与骨形成相关功能的有效调节因子，即有丝分裂、胶原蛋白合成和碱性磷酸酶活性。在TGF-β3和TGF-β1的直接比较中，TGF-β3的效力似乎比TGF-β1强三到五倍。我们用碘化TGF-β进行的交联实验表明，在富含成骨细胞的骨细胞培养物中，TGF-β3和TGF-β1都与相同的三个细胞表面结合位点相关。受体竞争研究的Scatchard分析表明，存在皮摩尔范围内TGF-β3的高亲和力结合位点。在总体结合中，TGF-β3的表观亲和力比TGF-β1高约四倍。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d395/361033/3ead5c4078a2/molcellb00045-0049-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d395/361033/7665f864cf2c/molcellb00045-0049-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d395/361033/3ead5c4078a2/molcellb00045-0049-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d395/361033/7665f864cf2c/molcellb00045-0049-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d395/361033/3ead5c4078a2/molcellb00045-0049-b.jpg

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