Graduate School of Biomedical Engineering, The University of New South Wales, Sydney, New South Wales 2052, Australia.
Biomaterials. 2011 Oct;32(28):6655-62. doi: 10.1016/j.biomaterials.2011.05.062. Epub 2011 Jun 14.
Chitosan has been shown to promote initial wound closure events to prevent blood loss. Platelet adhesion and activation are crucial early events in these processes after traumatic bleeding leading to thrombus formation. Platelet adhesion to chitosan was found to be enhanced in the presence of adsorbed plasma and extracellular matrix proteins and was found to be primarily mediated by α(IIb)β(3) integrins, while α(2)β(1) integrins were found to be involved in platelet adhesion to collagen and perlecan. Platelets were found to be activated by chitosan, as shown by an increase in the expression of α(IIb)β(3) integrins and P-selectin, while the extent of activation was modulated by the presence of proteins including perlecan and fibrinogen. Collagen-coated chitosan was found to activate platelets to the same extent as either chitosan or collagen alone. These data support the role of plasma and extracellular matrix proteins in promoting chitosan mediated platelet adhesion and activation supporting the hypothesis that chitosan promotes wound healing via these interactions.
壳聚糖已被证明可以促进初始伤口闭合事件，以防止失血。血小板黏附和激活是创伤性出血后血栓形成过程中的关键早期事件。研究发现，在吸附的血浆和细胞外基质蛋白存在的情况下，壳聚糖促进血小板黏附，并且主要由α(IIb)β(3)整合素介导，而α(2)β(1)整合素则参与血小板对胶原蛋白和 perlecan 的黏附。研究发现壳聚糖可激活血小板，表现为α(IIb)β(3)整合素和 P-选择素的表达增加，而激活程度可通过包括 perlecan 和纤维蛋白原在内的蛋白质的存在进行调节。研究发现，涂有胶原蛋白的壳聚糖激活血小板的程度与壳聚糖或胶原蛋白本身相同。这些数据支持血浆和细胞外基质蛋白在促进壳聚糖介导的血小板黏附和激活中的作用，支持壳聚糖通过这些相互作用促进伤口愈合的假说。
