Bodega Marine Laboratory and Section of Molecular & Cellular Biology University of California (Davis), Bodega Bay, California 94923.
Integr Comp Biol. 2005 Nov;45(5):715-24. doi: 10.1093/icb/45.5.715.
Encysted embryos (cysts) of the primitive crustacean, Artemia franciscana, are among the most resistant of all animal life history stages to extremes of environmental stress. These embryos, extremophiles of the animal kingdom, are the main focus of this paper. Previous work has revealed the importance of biochemical and biophysical adaptations that provide a significant part of the basis of their resistance, and I consider some of these here. In the present paper the critical role played by the outer layer of the shell in desiccation tolerance will be one focus. Another involves studies on the response of dried cysts to high temperatures that, among other things, implicate one or more volatile factors released from the cysts that determines the extent of thermotolerance under a given heating regime. A hypothetical scheme is given to account for these peculiar results. Based on western immunoblotting analysis, and data from the literature, the scheme also implicates the heat-induced translocation of the stress protein p26 to nuclei as a potential cause of the reduction in hatching level.
被囊化的丰年虾胚胎（囊）是所有动物生活史阶段中对环境压力极端条件最具抗性的之一。这些胚胎是动物王国中的极端微生物，是本文的主要关注点。之前的工作已经揭示了生化和生物物理适应的重要性，这些适应为它们的抗性提供了重要的基础，我在这里考虑了其中的一些。在本文中，外壳的外层在耐受干燥方面所起的关键作用将是一个重点。另一个涉及对干燥囊的高温响应的研究，除其他外，这涉及从囊释放的一种或多种挥发性因素，这些因素决定了在给定加热条件下的耐热程度。提出了一个假设方案来解释这些特殊的结果。基于西方免疫印迹分析以及文献中的数据，该方案还暗示应激蛋白 p26向核的热诱导易位可能是孵化水平降低的潜在原因。
