Finch D M, Jackson M B
Department of Neurology, University of California, Los Angeles 90024.
Brain Res. 1990 Jun 4;518(1-2):269-73. doi: 10.1016/0006-8993(90)90979-l.
The calcium-diacyglycerol activated protein kinase (C kinase) plays an important role in synaptic plasticity, and possibly in long-term potentiation (LTP). We have found that phorbol esters, which activate this protein kinase, increase the frequency of miniature excitatory postsynaptic currents (mEPCs) in hippocampal cell cultures. An analysis of distributions of these miniature synaptic currents shows that amplitudes, and thus postsynaptic function, are not influenced by phorbol esters. Synaptic transmission is therefore enhanced by phorbol esters at a presynaptic locus. The method of analysis employed here is applicable to a broad range of situations involving modification of synaptic transmission in the mammalian central nervous system.
钙 - 二酰甘油激活蛋白激酶（C激酶）在突触可塑性中发挥重要作用，可能也参与长时程增强（LTP）。我们发现，激活这种蛋白激酶的佛波酯可增加海马细胞培养物中微小兴奋性突触后电流（mEPCs）的频率。对这些微小突触电流分布的分析表明，其幅度以及突触后功能不受佛波酯影响。因此，佛波酯在突触前位点增强了突触传递。本文采用的分析方法适用于广泛的涉及哺乳动物中枢神经系统突触传递修饰的情况。
