Department of Pathology, University of Cambridge, Cambridge, UK.
J Med Virol. 2011 Aug;83(8):1345-50. doi: 10.1002/jmv.22111.
Condylomata acuminata (genital warts) are the most common sexually transmitted viral diseases. These lesions are caused by infection with mucosal human papillomaviruses (HPVs). However, there is limited information on HPV strain distribution involved in the molecular pathogenesis of these lesions. To address this, the strain prevalence and the frequency of multiple HPV infections were determined in wart tissue obtained from 31 patients attending a wart clinic. These lesions were bisected and subjected to parallel DNA and mRNA extractions. HPV-type prevalence and incidence of multiple infections were determined by the Roche Linear Array assay. qPCR compared HPV 6, 11, 16, and 18 viral loads and RT-qPCR measured HPV 6 and 11 E6 genomic expression levels. Seventy-one percent of these samples were infected with multiple HPVs. Only one sample was negative for HPV 6 or 11 DNA. Forty-eight percent of samples were positive for a high risk (oncogenic) HPV. The results show that multiple infections in tissue are frequent and the subsequent analysis of HPV 6 and 11 E6 DNA viral loads suggested that other HPVs could be causing lesions. Further analysis of HPV 6/11 E6 mRNA levels showed that there was no discernable relationship between HPV 6 E6 DNA viral load and relative HPV 6 or 11 E6 mRNA levels thereby questioning the relevance of viral load to lesion causality.
尖锐湿疣（生殖器疣）是最常见的性传播病毒疾病。这些病变是由黏膜人乳头瘤病毒（HPV）感染引起的。然而，关于参与这些病变分子发病机制的 HPV 株分布的信息有限。为了解决这个问题，在疣诊所就诊的 31 名患者的疣组织中确定了 HPV 株流行率和多重 HPV 感染的频率。这些病变被对半切开，并进行平行的 DNA 和 mRNA 提取。HPV 型流行率和多重感染的发生率通过罗氏线性阵列测定确定。qPCR 比较了 HPV 6、11、16 和 18 的病毒载量，RT-qPCR 测量了 HPV 6 和 11 E6 基因组表达水平。这些样本中有 71%感染了多种 HPV。只有一个样本 HPV 6 或 11 DNA 检测结果为阴性。48%的样本呈高危（致癌）HPV 阳性。结果表明，组织中的多重感染很常见，随后对 HPV 6 和 11 E6 DNA 病毒载量的分析表明，其他 HPV 可能导致病变。进一步分析 HPV 6/11 E6 mRNA 水平表明，HPV 6 E6 DNA 病毒载量与相对 HPV 6 或 11 E6 mRNA 水平之间没有明显关系，从而质疑病毒载量与病变因果关系的相关性。
