Muriel P, Mourelle M
Departamento de Farmacología y Toxicología, Centro de Investigacíon y de Estudios Avanzados del Instituto Politécnico Nacional, México, D.F.
J Appl Toxicol. 1990 Aug;10(4):281-4. doi: 10.1002/jat.2550100409.
The activities of Ca2(+)- and Na+, K(+)-ATPases were studied in liver plasma membranes from CCl4-cirrhotic rats and from livers of rats treated with silymarin in addition to CCl4. CCl4 chronic treatment produced significant decreases in Na+, K(+)- and Ca2(+)-ATPase activities; however, the animals treated with silymarin along with CCl4 showed no differences in ATPase activities as compared to controls. The lipid analysis performed in plasma membranes revealed increases in the cholesterol/phospholipid (CH/PL) and sphingomyelin/phosphatidylcholine (SM/PC) ratios in the cirrhotic group. Again, the membranes isolated from rats receiving CCl4 + silymarin showed normal CH/PL and SM/PC values. Considering that CH/PL and SM/PC ratios are related to membrane microviscosity, this study suggests that a lower fluidity of the membrane may be responsible for the observed decreases in ATPase activities in the cirrhotic group. Additionally, the role of silymarin to improve liver function in CCl4-cirrhosis can be attributed partially to its action at membrane level by preventing the increases in CH/PL and SM/PC ratios.
研究了四氯化碳诱导肝硬化大鼠以及除四氯化碳外还接受水飞蓟宾治疗的大鼠肝脏质膜中Ca2(+)-ATP酶和Na+, K(+)-ATP酶的活性。四氯化碳长期处理导致Na+, K(+)-ATP酶和Ca2(+)-ATP酶活性显著降低；然而，与对照组相比，同时接受水飞蓟宾和四氯化碳处理的动物在ATP酶活性方面没有差异。对质膜进行的脂质分析显示，肝硬化组的胆固醇/磷脂（CH/PL）和鞘磷脂/磷脂酰胆碱（SM/PC）比值升高。同样，从接受四氯化碳 + 水飞蓟宾处理的大鼠分离的质膜显示CH/PL和SM/PC值正常。鉴于CH/PL和SM/PC比值与膜微粘度有关，本研究表明膜流动性降低可能是肝硬化组中观察到的ATP酶活性降低的原因。此外，水飞蓟宾在四氯化碳诱导的肝硬化中改善肝功能的作用可部分归因于其通过防止CH/PL和SM/PC比值升高而在膜水平发挥的作用。
