Syrjänen S, Kärjä V, Chang F J, Johansson B, Syrjänen K
Department of Pathology and Forensic Medicine, University of Kuopio, Finland.
ORL J Otorhinolaryngol Relat Spec. 1990;52(4):254-9. doi: 10.1159/000276145.
Recently, Epstein-Barr virus (EBV) has gained increasing attention as a potential causative agent of Sjögren's syndrome, which is an autoimmune disorder characterized by lymphocytic infiltration of salivary and lacrimal glands. To determine the association between Sjögren's syndrome and EBV, we re-evaluated the salivary gland biopsies from patients previously diagnosed to have Sjögren's syndrome. Altogether, 20 cases of Sjögren's syndrome were reviewed from the files of the University Central Hospital since 1975. After re-evaluation of these biopsies, however, 11 patients did not fulfil the current criteria of Sjögren's syndrome, but were diagnosed as having chronic sialadenitis. All biopsies were processed for in situ hybridization (ISH) and polymerase chain reaction (PCR) to detect EBV DNA. With ISH, all samples were negative for EBV DNA. With PCR, however, 9 biopsies proved to be positive for EBV. 3 additional positive cases were found when the amplification product was dot-blotted and hybridized with EBV DNA probe. 10 of the EBV-positive salivary gland biopsies showed the histological features of chronic sialadenitis and only 2 cases were classified as having Sjögren's syndrome. On the basis of the present results, the association between EBV and Sjögren's syndrome remains doubtful.
最近，作为干燥综合征（一种以唾液腺和泪腺淋巴细胞浸润为特征的自身免疫性疾病）的潜在致病因子，爱泼斯坦-巴尔病毒（EBV）受到了越来越多的关注。为了确定干燥综合征与EBV之间的关联，我们重新评估了先前被诊断为患有干燥综合征患者的唾液腺活检样本。自1975年以来，我们从大学中心医院的档案中总共回顾了20例干燥综合征病例。然而，在对这些活检样本进行重新评估后，有11名患者不符合目前干燥综合征的诊断标准，而是被诊断为患有慢性涎腺炎。所有活检样本均进行原位杂交（ISH）和聚合酶链反应（PCR）以检测EBV DNA。ISH检测结果显示，所有样本的EBV DNA均为阴性。然而，PCR检测显示，9份活检样本的EBV呈阳性。当扩增产物进行斑点印迹并与EBV DNA探针杂交时，又发现了3例阳性病例。10份EBV阳性的唾液腺活检样本表现出慢性涎腺炎的组织学特征，只有2例被归类为干燥综合征。基于目前的结果，EBV与干燥综合征之间的关联仍值得怀疑。
