Department of Biomedical Engineering, Tufts University, Medford, Massachusetts 02155, USA.
J Biomed Mater Res A. 2011 Sep 15;98(4):567-75. doi: 10.1002/jbm.a.33149. Epub 2011 Jun 16.
Tissue sealants have emerged in recent years as strong candidates for hemostasis. A variety of formulations are currently commercially available and though they satisfy many of the markets' needs there are still key aspects of each that need improvement. Here we present a new class of blends, based on silk fibroin and chemically active polyethylene glycols (PEGs) with strong adhesive properties. These materials are cytocompatible, crosslink within seconds via chemical reaction between thiols and maleimides present on the constituent PEGs and have the potential to further stabilize through β-sheet formation by silk. Based on the silk concentration in the final formulation, the adhesive properties of these materials are comparable or better than the current leading PEG-based sealant. In addition, the silk-PEG based materials show decreased swelling and longer degradation times. Such properties would make them suitable for applications for which the current sealants are contraindicated.
近年来，组织粘合剂作为止血的有力候选者出现。目前有多种配方可在商业上获得，尽管它们满足了市场的许多需求，但每种配方仍有一些关键方面需要改进。在这里，我们提出了一类新的混合物，基于丝素纤维和具有强粘附性的化学活性聚乙二醇（PEG）。这些材料具有细胞相容性，通过存在于组成 PEG 上的巯基和马来酰亚胺之间的化学反应在几秒钟内交联，并且通过丝素形成β-折叠有进一步稳定的潜力。基于最终配方中的丝素浓度，这些材料的粘附性能与目前领先的基于 PEG 的密封剂相当或更好。此外，丝素-PEG 基材料显示出减少的肿胀和更长的降解时间。这些特性将使它们适用于当前密封剂被禁用的应用。
