Universidade Estadual de Londrina, Londrina, PR, Brazil.
Injury. 2012 Mar;43(3):284-9. doi: 10.1016/j.injury.2011.05.036.
The purpose of this work is to investigate the effects of simvastatin on sciatic nerve regeneration in male Wistar Rats.
Forty animals were allocated into four groups: (1) control (C); (2) control+simvastatin (CS); (3) lesioned animals+sterile PBS (LC) and (4) lesioned animals+simvastatin (LS). Lesioned animals were submitted to crushing lesion of right sciatic nerve. Simvastatin (20mg/kg/day, i.p.) was administered for five days. Footprints were obtained weekly for evaluation of functional locomotor recovery by means of the Sciatic Function Index (SFI). Blood samples were obtained weekly for quantifying circulating leukocytes. Animals were sacrificed after 21 days for histological analyses of sciatic nerve and spleen.
LS Animals presented increased SFI scores, decreased areas of oedema and mononuclear cell infiltration during Wallerian degeneration and nerve regeneration (7,14 and 21 days; P<0.05). Spleen weight and white pulp areas was increased in LC animals after 21 days. Increased numbers of circulating neutrophils were observed in simvastatin treated animals (CS e LS) at seven, 14 and 21 days, compared to non-treated groups (C and LC).
The study suggests that simvastatin accelerates the morphological and functional recovery process of the peripheral nervous system interfering with innate and acquired immunity.
本研究旨在探讨辛伐他汀对雄性 Wistar 大鼠坐骨神经再生的影响。
将 40 只动物分为 4 组：（1）对照组（C）；（2）对照组+辛伐他汀（CS）；（3）损伤动物+无菌 PBS（LC）和（4）损伤动物+辛伐他汀（LS）。损伤动物接受右侧坐骨神经挤压伤。辛伐他汀（20mg/kg/天，腹腔注射）连续给药 5 天。每周进行足迹实验以通过坐骨神经功能指数（SFI）评估功能运动恢复情况。每周采集血样以定量检测循环白细胞。伤后 21 天处死动物，对坐骨神经和脾脏进行组织学分析。
LS 动物的 SFI 评分升高，Wallerian 变性和神经再生过程中的水肿和单核细胞浸润面积减小（7、14 和 21 天；P<0.05）。LC 动物伤后 21 天脾脏重量和白髓面积增加。与未治疗组（C 和 LC）相比，辛伐他汀治疗组（CS 和 LS）在 7、14 和 21 天观察到循环中性粒细胞数量增加。
该研究表明，辛伐他汀通过干扰固有和获得性免疫加速周围神经系统的形态和功能恢复过程。
