Biomedical Engineering Institute, School of Control Science and Engineering, Shandong University, Jinan, 250021 Shandong, China.
Mol Biol Rep. 2012 Mar;39(3):3009-15. doi: 10.1007/s11033-011-1063-0. Epub 2011 Jun 19.
In order to determine the biological roles of the inhibitor of DNA-binding-1/inhibitor of differentiation-1 (ID-1) protein in MGC803 and AGS cell lines, we ectopically expressed or downregulated ID-1 in the both gastric cell lines and measured various parameters of tumor cell development, including cell proliferation, cell cycle progression, apoptosis and cell migration. The ectopic expression of ID-1 significantly enhanced cell proliferation, cell cycle progression and cell migration, and protected MGC803 and AGS cell lines from cisplatin-induced apoptosis. The opposite effects were observed after downregulation of ID-1, which in combination with cisplatin treatment enhanced apoptosis in a synergistic fashion. Collectively, these findings demonstrate that ID-1 plays pivotal and diverse roles in the biology of certain gastric cancer cells, further suggesting that ID-1 is implicated in the pathogenesis and progression of gastric cancer.
为了确定 DNA 结合抑制因子-1/分化抑制因子-1（ID-1）蛋白在 MGC803 和 AGS 细胞系中的生物学作用，我们在这两种胃细胞系中外源表达或下调 ID-1，并测量了肿瘤细胞发展的各种参数，包括细胞增殖、细胞周期进程、细胞凋亡和细胞迁移。ID-1 的异位表达显著增强了细胞增殖、细胞周期进程和细胞迁移，并保护 MGC803 和 AGS 细胞系免受顺铂诱导的细胞凋亡。下调 ID-1 后则观察到相反的效果，与顺铂联合处理后以协同方式增强了细胞凋亡。总之，这些发现表明 ID-1 在某些胃癌细胞的生物学中发挥着关键和多样化的作用，进一步表明 ID-1 与胃癌的发病机制和进展有关。
