Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
Biophys J. 2011 Jun 22;100(12):L63-5. doi: 10.1016/j.bpj.2011.05.020.
We report superresolution fluorescence microscopy in an intact living organism, namely Caenorhabditis elegans nematodes expressing green fluorescent protein (GFP)-fusion proteins. We also superresolve, by stimulated emission depletion (STED) microscopy, living cultured cells, demonstrating that STED microscopy with GFP can be widely applied. STED with GFP can be performed with both pulsed and continuous-wave lasers spanning a wide wavelength range from at least 556-592 nm. Acquiring subdiffraction resolution images within seconds enables the recording of movies revealing structural dynamics. These results demonstrate that numerous microscopy studies of live samples employing GFP as the marker can be performed at subdiffraction resolution.
我们在一个完整的活体生物中报告了超分辨率荧光显微镜，即表达绿色荧光蛋白（GFP）融合蛋白的秀丽隐杆线虫。我们还通过受激发射损耗（STED）显微镜对活培养细胞进行了超分辨，证明了 GFP 的 STED 显微镜可以广泛应用。 GFP 的 STED 可以使用从至少 556-592nm 的宽波长范围的脉冲和连续波激光器进行。在几秒钟内获取亚衍射分辨率的图像，可以记录显示结构动力学的电影。这些结果表明，许多使用 GFP 作为标记的活样本的显微镜研究可以在亚衍射分辨率下进行。
