利用光致变色现象对活细胞进行双标记 STED 纳米显微镜检测。

Dual-label STED nanoscopy of living cells using photochromism.

机构信息

Max-Planck-Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany.

出版信息

Nano Lett. 2011 Sep 14;11(9):3970-3. doi: 10.1021/nl202290w. Epub 2011 Aug 8.

DOI:10.1021/nl202290w

Abstract

We demonstrate live-cell STED microscopy of two protein species using photochromic green fluorescent proteins as markers. The reversible photoswitching of two markers is implemented so that they can be discerned with a single excitation and STED wavelength and a single detection channel. Dual-label STED microscopy is shown in living mammalian cells.

摘要

我们使用光致变色绿色荧光蛋白作为标记物，展示了两种蛋白质在活细胞中的 STED 显微镜观察。通过可逆光开关实现了两种标记物的切换，使得它们可以在单个激发和 STED 波长以及单个检测通道下被区分开来。双标记 STED 显微镜在活的哺乳动物细胞中得到了应用。