Soomro S, Shousha S
Department of Histopathology, Charing Cross and Westminster Medical School, Charing Cross Hospital, London.
J Clin Pathol. 1990 Aug;43(8):671-4. doi: 10.1136/jcp.43.8.671.
Several immunohistological methods for the demonstration of progesterone receptors were tried on routinely processed paraffin wax sections of breast carcinoma, using Abbott's PgR-ICA monoclonal antibody. The best results were obtained with the avidin-biotin-immunoperoxidase complex method with no prior trypsinisation or DNAse digestion, and with imidazole added to the final diaminobenzidine developing solution. A simple semiquantitative scoring system was used to assess the staining results which were then compared with the results obtained by a standard dextran-coated charcoal biochemical assay. Of 31 cases examined, the results of the two methods were concordant in 25 (81%) of cases. This is near the higher end of the concordance range obtained by several other authors using frozen sections. The discordance encountered in a few cases was possibly the result of sampling errors which are more likely to occur with the chemical rather than the histological method. It is concluded that the method described here is fairly reliable and would greatly simplify the process of assessment of progesterone receptors in breast, and possibly other tumours.
使用雅培公司的PgR-ICA单克隆抗体，在常规处理的乳腺癌石蜡切片上尝试了几种用于检测孕激素受体的免疫组织学方法。采用抗生物素蛋白-生物素-免疫过氧化物酶复合物方法，且不预先进行胰蛋白酶消化或DNA酶消化，并在最终的二氨基联苯胺显影液中添加咪唑，可获得最佳结果。使用简单的半定量评分系统评估染色结果，然后将其与通过标准葡聚糖包被活性炭生化分析获得的结果进行比较。在检查的31例病例中，两种方法的结果在25例（81%）病例中一致。这接近其他几位作者使用冰冻切片获得的一致性范围的较高端。少数病例中出现的不一致可能是抽样误差的结果，化学方法比组织学方法更易出现这种误差。得出的结论是，这里描述的方法相当可靠，将极大地简化乳腺及可能其他肿瘤中孕激素受体的评估过程。
