Shousha S, Coady A T, Stamp T, James K R, Alaghband-Zadeh J
Department of Histopathology, Charing Cross Hospital, London.
J Clin Pathol. 1989 Sep;42(9):902-5. doi: 10.1136/jcp.42.9.902.
An immunohistological method (Shintaku-Said method) for the demonstration of oestrogen receptors in routinely processed paraffin wax embedded tissue was applied to 19 cases of mucinous carcinoma of the breast. Seventeen (89%) tumours showed variable degrees of positivity and two were negative. In eight cases the receptors were also assayed biochemically using a dextran-coated charcoal method, and the results of the two methods showed good correlation. No difference in the distribution of positive and negative cases was noted between pure and mixed mucinous tumours, and in the latter group the pattern of staining of the mucinous elements was similar to that seen in the solid elements. It is concluded that the major advantage of this method is its ability to offer for study the distribution of the receptors in individual cells and specific histological structures. The results also indicate that most mucinous carcinomas of the breast are oestrogen receptor positive, irrespective of whether they are pure or mixed type.
一种用于在常规处理的石蜡包埋组织中显示雌激素受体的免疫组织学方法（新宅-赛德法）应用于19例乳腺黏液癌。17例（89%）肿瘤显示不同程度的阳性，2例为阴性。8例还采用葡聚糖包被活性炭法进行了受体的生化检测，两种方法的结果显示出良好的相关性。在纯黏液性肿瘤和混合性黏液性肿瘤之间，阳性和阴性病例的分布没有差异，在后一组中，黏液成分的染色模式与实性成分相似。结论是，该方法的主要优点是能够研究受体在单个细胞和特定组织结构中的分布。结果还表明，大多数乳腺黏液癌雌激素受体呈阳性，无论其为纯型还是混合型。
