Chew Siew-Choo, Khor Geok-Lin, Loh Su-Peng
Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia..
J Nutr Sci Vitaminol (Tokyo). 2011;57(2):150-5. doi: 10.3177/jnsv.57.150.
Folate is of prime interest among investigators in nutrition due to its multiple roles in maintaining health, especially in preventing neural tube defects and reducing the risk of cardiovascular diseases. We investigated the effect of dietary folate intake on blood folate, vitamin B(12), vitamin B(6), and homocysteine status. One hundred subjects consisting of Chinese and Malay subjects volunteered to participate in this cross-sectional study. Dietary folate intake was assessed by 24-h dietary recall and a food-frequency questionnaire (FFQ). Serum and red blood cell folate were analyzed using a microbiological assay, while serum vitamin B(12) was determined by electrochemiluminescence immunoassay (ECLIA), and high-performance liquid chromatography (HPLC) was used for the determination of serum vitamin B(6) and homocysteine. The mean folate intake, serum folate, RBC folate, serum vitamin B(12), and B(6), were higher in female subjects, with the exception of serum homocysteine. The Chinese tended to have higher folate intake, serum folate, RBC folate, and vitamin B(12). A positive association was found between folate intake and serum folate while a negative association was found between folate intake and serum homocysteine. Stepwise linear regression of serum folate showed a significant positive coefficient for folate intake whilst a significant negative coefficient was found for serum homocysteine when controlling for age, gender, and ethnicity. In conclusion, high dietary folate intake helps to increase serum folate and to lower the homocysteine levels.
由于叶酸在维持健康方面具有多种作用，尤其是在预防神经管缺陷和降低心血管疾病风险方面，因此它在营养研究人员中备受关注。我们研究了膳食叶酸摄入量对血液叶酸、维生素B12、维生素B6和同型半胱氨酸水平的影响。100名由华裔和马来裔受试者组成的志愿者参与了这项横断面研究。通过24小时膳食回顾和食物频率问卷（FFQ）评估膳食叶酸摄入量。血清和红细胞叶酸采用微生物法分析，血清维生素B12采用电化学发光免疫分析法（ECLIA）测定，血清维生素B6和同型半胱氨酸采用高效液相色谱法（HPLC）测定。除血清同型半胱氨酸外，女性受试者的平均叶酸摄入量、血清叶酸、红细胞叶酸、血清维生素B12和B6较高。华裔受试者的叶酸摄入量、血清叶酸、红细胞叶酸和维生素B12往往较高。叶酸摄入量与血清叶酸之间呈正相关，而叶酸摄入量与血清同型半胱氨酸之间呈负相关。血清叶酸的逐步线性回归显示，在控制年龄、性别和种族后，叶酸摄入量的系数显著为正，而血清同型半胱氨酸的系数显著为负。总之，高膳食叶酸摄入量有助于提高血清叶酸水平并降低同型半胱氨酸水平。
