Department of Anatomy and Neurobiology, Washington University in St. Louis, St. Louis, MO 63110, USA.
Science. 2011 Jun 24;332(6037):1571-6. doi: 10.1126/science.1202249.
Sleep is believed to play an important role in memory consolidation. We induced sleep on demand by expressing the temperature-gated nonspecific cation channel Transient receptor potential cation channel (UAS-TrpA1) in neurons, including those with projections to the dorsal fan-shaped body (FB). When the temperature was raised to 31°C, flies entered a quiescent state that meets the criteria for identifying sleep. When sleep was induced for 4 hours after a massed-training protocol for courtship conditioning that is not capable of inducing long-term memory (LTM) by itself, flies develop an LTM. Activating the dorsal FB in the absence of sleep did not result in the formation of LTM after massed training.
睡眠被认为在记忆巩固中起着重要作用。我们通过在神经元中表达温度门控非特异性阳离子通道瞬时受体电位阳离子通道（UAS-TrpA1）来按需诱导睡眠，包括那些投射到背扇形体（FB）的神经元。当温度升高到 31°C 时，苍蝇进入一种静止状态，符合识别睡眠的标准。当在求偶条件反射的大量训练方案后诱导 4 小时的睡眠时，苍蝇会形成长期记忆（LTM）。大量训练后，在没有睡眠的情况下激活背 FB 并不能导致 LTM 的形成。
