Department of Medicinal Chemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi, 221005, India.
Inflammopharmacology. 2011 Oct;19(5):273-81. doi: 10.1007/s10787-011-0087-3. Epub 2011 Jun 24.
The leaves of Jasminum grandiflorum (JG) are in clinical use in Ayurveda for wound management. Since, oxidative stress and inflammation are the primary causes in delayed wound healing, so here its antioxidant and anti-inflammatory activities have been investigated using in vitro as well as in vivo models. The solvent-free methanolic extract of dried leaves of JG were tested for its trapping capacity toward pre-generated ABTS•+ radicals, instantly generated superoxide and hydroxyl radicals, along with metal chelation property, reducing power and total phenolic content. Further, it was tested on LPS-induced nitric oxide and cell viability, on primary culture of rat peritoneal macrophages. Its anti-inflammatory property was also tested on carrageenan-induced paw edema in rats. This extract significantly inhibited iron-induced lipid peroxidation and trapped ABTS•+, superoxide and OH radicals. It significantly inhibited nitric oxide (NO) release, without affecting the cell viability at 800 μg/ml concentration and reduced the formation of paw edema in rats. Thus, it could be suggested that the aforesaid anti-inflammatory properties of JG leaves are associated to its high phenolic content (2.25±0.105 mg/l of gallic acid equivalent), reducing power and its free radical-scavenging property.
素馨花的叶子在印度阿育吠陀医学中被用于临床伤口管理。由于氧化应激和炎症是延迟伤口愈合的主要原因，因此在这里使用体外和体内模型研究了其抗氧化和抗炎活性。用无溶剂甲醇提取干燥素馨花叶子的提取物，检测其对预先生成的 ABTS•+自由基、即时生成的超氧阴离子自由基和羟基自由基的捕获能力，以及金属螯合能力、还原能力和总酚含量。进一步，在脂多糖诱导的一氧化氮和大鼠原代腹腔巨噬细胞活力上进行检测。在大鼠角叉菜胶诱导的足肿胀模型中也对其抗炎作用进行了检测。该提取物能显著抑制铁诱导的脂质过氧化，捕获 ABTS•+、超氧阴离子自由基和羟基自由基。它能显著抑制一氧化氮（NO）的释放，在 800μg/ml 浓度下不影响细胞活力，并减少大鼠足肿胀的形成。因此，可以认为素馨花叶子的上述抗炎特性与其高酚含量（2.25±0.105mg/l 没食子酸当量）、还原能力和自由基清除能力有关。
