Dipartimento di Scienza e Tecnologia del Farmaco, University of Torino, Italy.
J Microencapsul. 2011;28(6):537-48. doi: 10.3109/02652048.2011.590615. Epub 2011 Jun 27.
Curcumin (CU) loaded solid lipid nanoparticles (SLNs) of fatty acids (FA) were prepared with a coacervation technique based on FA precipitation from their sodium salt micelles in the presence of polymeric non-ionic surfactants. Myristic, palmitic, stearic, and behenic acids, and different polymers with various molecular weights and hydrolysis grades were employed as lipid matrixes and stabilisers, respectively. Generally, spherical-shaped nanoparticles with mean diameters below 500 nm were obtained, and using only middle-high hydrolysis, grade-polymer SLNs with diameters lower than 300 nm were produced. CU encapsulation efficiency was in the range 28-81% and highly influenced by both FA and polymer type. Chitosan hydrochloride was added to FA SLN formulations to produce bioadhesive, positively charged nanoparticles. A CU-chitosan complex formation could be hypothesised by DSC analysis, UV-vis spectra and chitosan surface tension determination. A preliminary study on HCT-116 colon cancer cells was developed to evaluate the influence of CU-loaded FA SLNs on cell viability.
姜黄素（CU）负载的脂肪酸（FA）固体脂质纳米粒（SLNs）是通过凝聚技术制备的，该技术基于在聚合非离子表面活性剂存在下，从 FA 的钠盐胶束中沉淀 FA。肉豆蔻酸、棕榈酸、硬脂酸和山嵛酸，以及具有不同分子量和水解度的不同聚合物分别用作脂质基质和稳定剂。通常，得到平均直径低于 500nm 的球形纳米粒，并且仅使用中高水解度，可制得直径低于 300nm 的聚合物 SLNs。CU 包封效率在 28-81%范围内，受 FA 和聚合物类型的影响很大。盐酸壳聚糖被添加到 FA SLN 配方中，以制备具有生物黏附性的正电荷纳米粒。通过 DSC 分析、UV-vis 光谱和壳聚糖表面张力测定，可以假设 CU-壳聚糖复合物的形成。初步研究了 HCT-116 结肠癌细胞，以评估载 CU 的 FA SLNs 对细胞活力的影响。
