Ito T, Brill A B
Department of Nuclear Medicine, University of Massachusetts Medical Center, Worcester 01655.
Int J Rad Appl Instrum A. 1990;41(7):661-7. doi: 10.1016/0883-2889(90)90081-q.
A method of preparing radioactive standards for quantitative whole-body autoradiography (WBARG) using short-lived radionuclides is described and evaluated. Radioactive standards were prepared from bovine brain paste which was mixed with serial dilutions of the radionuclide being studied. Homogeneous distribution of the radionuclide in the brain paste was verified by direct counting and autoradiographic techniques. In addition, there was good agreement between the amounts of radioactivity obtained from quantitative WBARG and tissue counting in all of the major tissues sampled. Therefore, this method is effective for quantitative analysis of WBARG using short-lived radionuclides, for which permanent reference standards are unavailable.
描述并评估了一种使用短寿命放射性核素制备用于定量全身放射自显影（WBARG）的放射性标准品的方法。放射性标准品由牛脑糊制备，该牛脑糊与所研究放射性核素的系列稀释液混合。通过直接计数和放射自显影技术验证了放射性核素在脑糊中的均匀分布。此外，在所有采样的主要组织中，从定量WBARG获得的放射性活度与组织计数之间具有良好的一致性。因此，该方法对于使用短寿命放射性核素的WBARG定量分析是有效的，而对于短寿命放射性核素而言，无法获得永久性参考标准品。
