de Crecy-Lagard V, Glaser P, Lejeune P, Sismeiro O, Barber C E, Daniels M J, Danchin A
Unité de Régulation de l'Expression Génétique, Institut Pasteur, Paris, France.
J Bacteriol. 1990 Oct;172(10):5877-83. doi: 10.1128/jb.172.10.5877-5883.1990.
A DNA fragment from Xanthomonas campestris pv. campestris that partially restored the carbohydrate fermentation pattern of a cya crp Escherichia coli strain was cloned and expressed in E. coli. The nucleotide sequence of this fragment revealed the presence of a 700-base-pair open reading frame that coded for a protein highly similar to the catabolite activation factor (CAP) of E. coli (accordingly named CLP for CAP-like protein). An X. campestris pv. campestris clp mutant was constructed by reverse genetics. This strain was not affected in the utilization of various carbon sources but had strongly reduced pathogenicity. Production of xanthan gum, pigment, and extracellular enzymes was either increased or decreased, suggesting that CLP plays a role in the regulation of phytopathogenicity.
从野油菜黄单胞菌野油菜致病变种中分离出一个DNA片段，该片段可部分恢复cya crp大肠杆菌菌株的碳水化合物发酵模式，并在大肠杆菌中进行克隆和表达。该片段的核苷酸序列显示存在一个700个碱基对的开放阅读框，编码一种与大肠杆菌分解代谢激活因子（CAP）高度相似的蛋白质（因此命名为CLP，即类CAP蛋白）。通过反向遗传学构建了野油菜黄单胞菌野油菜致病变种clp突变体。该菌株在各种碳源的利用上未受影响，但致病性显著降低。黄原胶、色素和细胞外酶的产生要么增加要么减少，这表明CLP在植物致病性调控中发挥作用。
