Wlodkowic Donald, Darzynkiewicz Zbigniew
The BioMEMS Research Group, Department of Chemistry, University of Auckland, Auckland, New Zealand.
Methods Cell Biol. 2011;102:105-25. doi: 10.1016/B978-0-12-374912-3.00005-5.
The past decade has brought many innovations to the field of flow and image-based cytometry. These advancements can be seen in the current miniaturization trends and simplification of analytical components found in the conventional flow cytometers. On the other hand, the maturation of multispectral imaging cytometry in flow imaging and the slide-based laser scanning cytometers offers great hopes for improved data quality and throughput while proving new vistas for the multiparameter, real-time analysis of cells and tissues. Importantly, however, cytometry remains a viable and very dynamic field of modern engineering. Technological milestones and innovations made over the last couple of years are bringing the next generation of cytometers out of centralized core facilities while making it much more affordable and user friendly. In this context, the development of microfluidic, lab-on-a-chip (LOC) technologies is one of the most innovative and cost-effective approaches toward the advancement of cytometry. LOC devices promise new functionalities that can overcome current limitations while at the same time promise greatly reduced costs, increased sensitivity, and ultra high throughputs. We can expect that the current pace in the development of novel microfabricated cytometric systems will open up groundbreaking vistas for the field of cytometry, lead to the renaissance of cytometric techniques and most importantly greatly support the wider availability of these enabling bioanalytical technologies.
在过去十年里，流式细胞术和基于图像的细胞术领域取得了诸多创新。这些进展体现在当前传统流式细胞仪的小型化趋势以及分析组件的简化上。另一方面，流式成像和基于玻片的激光扫描细胞仪中多光谱成像细胞术的成熟，为提高数据质量和通量带来了巨大希望，同时也为细胞和组织的多参数实时分析开辟了新前景。然而，重要的是，细胞术仍然是现代工程中一个充满活力且可行的领域。过去几年取得的技术里程碑和创新成果，正使下一代细胞仪走出集中式核心设施，同时使其价格更亲民、使用更便捷。在此背景下，微流控芯片实验室（LOC）技术的发展是推动细胞术进步的最具创新性和成本效益的方法之一。LOC设备有望实现新功能，既能克服当前的局限性，又能大幅降低成本、提高灵敏度并实现超高通量。我们可以预期，新型微制造细胞术系统当前的发展速度将为细胞术领域开辟开创性的前景，引领细胞术技术的复兴，最重要的是，极大地支持这些生物分析技术更广泛的应用。
