Vasudeva Neeru, Vats Manisha
Division of Pharmacognosy, Department of Pharmaceutical Sciences, Guru Jambheshwar University of Science and Technology, Haryana, India.
J Acupunct Meridian Stud. 2011 Jun;4(2):116-22. doi: 10.1016/S2005-2901(11)60017-4.
The study was undertaken to evaluate the anti-spermatogenic efficacy of ethanol extract of stem bark of Dalbergia sissoo Roxb. For the in vitro study, semen samples were obtained from 15 healthy fertile men aged 25-35 years. Sperm motility was examined by the Sander-Cramer method. A dose-dependent and time-dependent effect of ethanol extract on sperm motility and sperm viability were observed. Various concentrations affected the motility of sperm. Ethanol extract at a concentration of 20 mg/mL caused complete immobilization within 3 minutes. Sperm viability and hypo-osmotic swelling was significantly reduced at this concentration. The in vivo studies were carried out on Swiss male albino mice. Ethanol extract at a dose of 200 mg/kg body weight resulted in a significant decrease (p<0.001) in weight of the testis and epididymis. A significant decrease (p<0.01) in sperm motility and sperm count in the epididymis were observed. Histological changes in the epididymis and testis were also investigated.
本研究旨在评估印度黄檀茎皮乙醇提取物的抗生精功效。在体外研究中，从15名年龄在25至35岁的健康可育男性获取精液样本。采用桑德 - 克莱默法检测精子活力。观察到乙醇提取物对精子活力和精子存活率具有剂量依赖性和时间依赖性效应。不同浓度影响精子活力。浓度为20mg/mL的乙醇提取物在3分钟内导致精子完全失活。在此浓度下，精子存活率和低渗肿胀率显著降低。体内研究在瑞士雄性白化小鼠身上进行。体重200mg/kg的乙醇提取物导致睾丸和附睾重量显著降低（p<0.001）。观察到附睾中精子活力和精子数量显著减少（p<0.01）。还研究了附睾和睾丸的组织学变化。
