Mannan Md Abdul, Khatun Ambia, Khan Md Farhad Hossen
Department of Pharmacy, Stamford University Bangladesh, 51, Siddeswari Road, Dhaka, 1217, Bangladesh.
BMC Complement Altern Med. 2017 Jan 23;17(1):72. doi: 10.1186/s12906-017-1565-y.
Dalbergia sissoo DC. (Family: Fabaceae) is a medium to large deciduous tree, is locally called "shishu" in Bangladesh. It is used to treat sore throats, dysentery, syphilis, bronchitis, inflammations, infections, hernia, skin diseases, and gonorrhea. This study evaluated the antinociceptive effect of the methanol extract of D. sissoo leaves (MEDS) in mice.
The extract was assessed for antinociceptive activity using chemical and heat induced pain models such as hot plate, tail immersion, acetic acid-induced writhing, formalin, glutamate, and cinnamaldehyde test models in mice at the doses of 100, 200, and 400 mg/kg (p.o.) respectively. Morphine sulphate (5 mg/kg, i.p.) and diclofenac sodium (10 mg/kg, i.p.) were used as reference analgesic drugs. To confirm the possible involvement of opioid receptor in the central antinociceptive effect of MEDS, naloxone was used to antagonize the effect.
MEDS demonstrated potent and dose-dependent antinociceptive activity in all the chemical and heat induced mice models (p < 0.001). The findings of this study indicate that the involvement of both peripheral and central antinociceptive mechanisms. The use of naloxone verified the association of opioid receptors in the central antinociceptive effect.
This study indicated the peripheral and central antinociceptive activity of the leaves of D. sissoo. These results support the traditional use of this plant in different painful conditions.
印度黄檀（豆科）是一种中型至大型落叶乔木，在孟加拉国当地被称为“shishu”。它被用于治疗喉咙痛、痢疾、梅毒、支气管炎、炎症、感染、疝气、皮肤病和淋病。本研究评估了印度黄檀叶甲醇提取物（MEDS）对小鼠的抗伤害感受作用。
分别以100、200和400毫克/千克（口服）的剂量，使用化学和热诱导疼痛模型，如热板法、尾浸法、醋酸诱导扭体法、福尔马林法、谷氨酸法和肉桂醛试验模型，对提取物的抗伤害感受活性进行评估。硫酸吗啡（5毫克/千克，腹腔注射）和双氯芬酸钠（10毫克/千克，腹腔注射）用作参考镇痛药。为了证实阿片受体可能参与MEDS的中枢抗伤害感受作用，使用纳洛酮来拮抗这种作用。
MEDS在所有化学和热诱导的小鼠模型中均表现出强效且剂量依赖性的抗伤害感受活性（p < 0.001）。本研究结果表明，外周和中枢抗伤害感受机制均有参与。纳洛酮的使用证实了阿片受体与中枢抗伤害感受作用之间的关联。
本研究表明印度黄檀叶具有外周和中枢抗伤害感受活性。这些结果支持了这种植物在不同疼痛病症中的传统用途。
