Auvinen P, Hyypiä T
Department of Virology, University of Turku, Finland.
J Gen Virol. 1990 Sep;71 ( Pt 9):2133-9. doi: 10.1099/0022-1317-71-9-2133.
We have studied the genetic relationships of echoviruses using nucleotide sequencing and hybridization analysis. The nucleotide sequence of the echovirus 11 (EV11) P2 and P3 regions, which encode the nonstructural proteins, was shown to resemble closely those of coxsackie B viruses (CBV) and coxsackievirus A9 (CAV9). EV11, CBV and CAV9 have a similar organization in the 3' non-coding region when compared to polioviruses and CAV21. In contrast, the 3' end of EV22 shares only minimal sequence homology with other sequenced enteroviruses, and the 3' non-coding region has a unique secondary structure. Thirty-three echovirus reference strains were tested by nucleic acid hybridization using cDNA probes from the genomes of EV6, 11, 18 and 22. It was shown that a great majority of the strains belongs to the same subgroup as serotypes 6, 11 and 18, whereas EV22 and EV23 are genetically not closely related to this major subgroup.
我们利用核苷酸测序和杂交分析研究了埃可病毒的遗传关系。编码非结构蛋白的埃可病毒11型（EV11）P2和P3区域的核苷酸序列显示与柯萨奇B病毒（CBV）和柯萨奇A9病毒（CAV9）的核苷酸序列非常相似。与脊髓灰质炎病毒和CAV21相比，EV11、CBV和CAV9在3'非编码区具有相似的结构。相比之下，EV22的3'端与其他已测序肠道病毒仅具有最小的序列同源性，并且3'非编码区具有独特的二级结构。使用来自EV6、11、18和22基因组的cDNA探针，通过核酸杂交对33株埃可病毒参考菌株进行了检测。结果表明，绝大多数菌株与血清型6、11和18属于同一亚组，而EV22和EV23在遗传上与这个主要亚组关系不密切。
