Department of Gynecology and Obstetrics, West China Second Hospital, Sichuan University, Chengdu, China.
Sci China Life Sci. 2011 Jun;54(6):502-12. doi: 10.1007/s11427-011-4177-7. Epub 2011 Jun 26.
Viruses replicate and proliferate in host cells while continuously adjusting to and modulating the host environment. They encode a wide spectrum of multifunctional proteins, which interplay with and modify proteins in host cells. Viral genomes were chronologically the first to be sequenced. However, the corresponding viral proteomes, the alterations of host proteomes upon viral infection, and the dynamic nature of proteins, such as post-translational modifications, enzymatic cleavage, and activation or destruction by proteolysis, remain largely unknown. Emerging high-throughput techniques, in particular quantitative or semi-quantitative mass spectrometry-based proteomics analysis of viral and cellular proteomes, have been applied to define viruses and their interactions with their hosts. Here, we review the major areas of viral proteomics, including virion proteomics, structural proteomics, viral protein interactomics, and changes to the host cell proteome upon viral infection.
病毒在宿主细胞中复制和增殖，同时不断适应和调节宿主环境。它们编码广泛的多功能蛋白，这些蛋白与宿主细胞中的蛋白相互作用并对其进行修饰。病毒基因组是最早被测序的基因组。然而，相应的病毒蛋白质组、病毒感染后宿主蛋白质组的改变，以及蛋白质的动态性质，如翻译后修饰、酶切、激活或蛋白酶降解，在很大程度上仍然未知。新兴的高通量技术，特别是基于定量或半定量质谱的病毒和细胞蛋白质组学分析，已被应用于定义病毒及其与宿主的相互作用。在这里，我们综述了病毒蛋白质组学的主要领域，包括病毒粒子蛋白质组学、结构蛋白质组学、病毒蛋白相互作用组学以及病毒感染后宿主细胞蛋白质组的变化。
