Vaccine & Gene Therapy Institute, Oregon Health & Science University, 505 NW 185th Avenue, Beaverton, OR 97006, USA.
Future Microbiol. 2010 Feb;5(2):303-11. doi: 10.2217/fmb.09.121.
The recent development of RNAi-based techniques for protein knockdown in mammalian cells has allowed for unprecedented flexibility in the study of protein function. Currently, large siRNA libraries are available that allow the knockdown of all proteins known to be encoded by the human genome. These libraries have been used to identify the host proteins required for the replication of several clinically important viruses, including HIV, flaviviruses and influenza. This review summarizes the methods used in RNAi-based screening for host factors involved in virus replication, and discusses published examples of such screens.
近年来，基于 RNAi 的技术在哺乳动物细胞中实现了蛋白质的敲低，这为研究蛋白质功能提供了前所未有的灵活性。目前，已经有大量的 siRNA 文库可供使用，这些文库可以实现对已知由人类基因组编码的所有蛋白质的敲低。这些文库已被用于鉴定几种临床重要病毒（包括 HIV、黄病毒和流感病毒）复制所必需的宿主蛋白。本文综述了基于 RNAi 的宿主因子筛选方法在病毒复制中的应用，并讨论了已发表的此类筛选的例子。
