J Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD 20850, USA.
Future Microbiol. 2010 Feb;5(2):289-301. doi: 10.2217/fmb.10.7.
While high-throughput protein-protein interaction screens were first published approximately 10 years ago, systematic attempts to map interactions among viruses and hosts started only a few years ago. HIV-human interactions dominate host-pathogen interaction databases (with approximately 2000 interactions) despite the fact that probably none of these interactions have been identified in systematic interaction screens. Recently, combinations of protein interaction data with RNAi and other functional genomics data allowed researchers to model more complex interaction networks. The rapid progress in this area promises a flood of new data in the near future, with clinical applications as soon as structural and functional genomics catches up with next-generation sequencing of human variation and structure-based drug design.
虽然高通量蛋白质-蛋白质相互作用筛选技术大约在 10 年前首次发表，但系统地绘制病毒和宿主之间相互作用的尝试直到近几年才开始。尽管在系统相互作用筛选中可能没有发现这些相互作用中的任何一种，但 HIV-人类相互作用仍然主导着宿主-病原体相互作用数据库（约有 2000 种相互作用）。最近，将蛋白质相互作用数据与 RNAi 和其他功能基因组学数据相结合，使研究人员能够构建更复杂的相互作用网络。该领域的快速发展有望在不久的将来涌现出大量新数据，随着结构和功能基因组学赶上基于下一代测序的人类变异和基于结构的药物设计，临床应用也将随之而来。
