Duan Y P, Chen X Z, Zhang X, Liu D G, Xia S S
Institute of Organ Transplantation, Tongji Medical University, Wuhan.
J Tongji Med Univ. 1990;10(2):104-10. doi: 10.1007/BF02887871.
Urine and blood specimens from 32 recipients of allograft organ transplant were investigated for human cytomegalovirus (HCMV) by conventional cell culture, indirect immunofluorescent assay subsequent to the rapid cell culture and DNA-DNA hybridization. The results showed that the rapid cell culture technique might be the best method for rapid detection of active HCMV infection after transplantation because it possesses the advantages of simplicity, speediness, sensitiveness, and high reliability of detecting productive HCMV infection. The major symptoms were prolonged or intermittent fever unresponsive to antibiotics, dysfunction or loss of function of the transplanted organ, pulmonary infiltrates and leukopenia. As our results suggested, reactivation of a latent infection appears to be the most probable etiologic factor contributing to HCMV infection after transplantation.
采用传统细胞培养、快速细胞培养后的间接免疫荧光测定法以及DNA-DNA杂交技术，对32例同种异体器官移植受者的尿液和血液标本进行了人巨细胞病毒（HCMV）检测。结果表明，快速细胞培养技术可能是移植后快速检测活动性HCMV感染的最佳方法，因为它具有操作简单、速度快、灵敏度高以及检测HCMV增殖性感染可靠性高的优点。主要症状为抗生素治疗无效的持续性或间歇性发热、移植器官功能障碍或功能丧失、肺部浸润和白细胞减少。正如我们的结果所示，潜伏感染的激活似乎是移植后导致HCMV感染的最可能病因。
