Prösch S, Kimel V, Dawydowa I, Krüger D H
Institute of Virology, Humboldt University Medical School (Charité), Berlin, Germany.
J Med Virol. 1992 Dec;38(4):246-51. doi: 10.1002/jmv.1890380404.
A modified centrifugation culture technique and a polymerase chain reaction (PCR) is described for detection of early antigen and IE antigen DNA, respectively, for rapid and sensitive monitoring of active cytomegalovirus (HCMV) infection after organ transplantation. In a preliminary study, 541 clinical specimens (blood, urine, bronchoalveolar lavage, pharyngeal wash, sputum) from 59 organ recipients were assayed for HCMV antigen by centrifugation culture; 144 samples were tested by PCR simultaneously. Antigenemia detected by centrifugation culture correlated strongly with active HCMV infection and clinical symptoms and proved useful for monitoring the efficacy of antiviral therapy. PCR was more sensitive in an earlier phase of infection when centrifugation culture was still negative. The clinical usefulness of both methods is discussed.
本文描述了一种改良的离心培养技术和聚合酶链反应（PCR），分别用于检测早期抗原和IE抗原DNA，以便对器官移植后活动性巨细胞病毒（HCMV）感染进行快速、灵敏的监测。在一项初步研究中，对59名器官接受者的541份临床标本（血液、尿液、支气管肺泡灌洗、咽拭子、痰液）进行了离心培养以检测HCMV抗原；同时对144份样本进行了PCR检测。通过离心培养检测到的抗原血症与活动性HCMV感染和临床症状密切相关，并证明对监测抗病毒治疗的疗效有用。在感染的早期阶段，当离心培养仍为阴性时，PCR更为灵敏。本文还讨论了这两种方法的临床实用性。
