Biological Soft Matter Group, FOM Institute AMOLF, Science Park 104, 1098 XG Amsterdam, The Netherlands.
Langmuir. 2011 Aug 16;27(16):10061-71. doi: 10.1021/la201604z. Epub 2011 Jul 12.
We demonstrate that cytoskeletal actin-myosin networks can be encapsulated with high efficiency in giant liposomes by hydration of lipids in an agarose hydrogel. The liposomes have cell-sized diameters of 10-20 μm and a uniform actin content. We show by measurements of membrane fluorescence intensity and bending rigidity that the majority of liposomes are unilamellar. We further demonstrate that the actin network can be specifically anchored to the membrane by biotin-streptavidin linkages. These protein-filled liposomes are useful model systems for quantitative studies of the physical mechanisms by which the cytoskeleton actively controls cell shape and mechanics. In a broader context, this new preparation method should be widely applicable to encapsulation of proteins and polymers, for instance, to create polymer-reinforced liposomes for drug delivery.
我们证明了通过琼脂糖水凝胶中脂质的水合作用，可以将细胞骨架肌动球蛋白网络高效地包裹在巨大的脂质体中。这些脂质体的直径为 10-20μm，具有均一的肌动蛋白含量，是典型的细胞膜大小。通过测量膜荧光强度和弯曲刚性，我们发现大多数脂质体都是单层的。我们进一步证明，肌动蛋白网络可以通过生物素-链霉亲和素键特异性地锚定在膜上。这些充满蛋白质的脂质体是定量研究细胞骨架主动控制细胞形状和力学的物理机制的有用模型系统。更广泛地说，这种新的制备方法应该广泛适用于蛋白质和聚合物的封装，例如，用于创建用于药物输送的聚合物增强型脂质体。
