可生物兼容的人工 DNA 连接子，可被 DNA 聚合酶读取，且在大肠杆菌中具有功能。
Biocompatible artificial DNA linker that is read through by DNA polymerases and is functional in Escherichia coli.
机构信息
School of Chemistry, University of Southampton, Highfield, Southampton SO17 1BJ, United Kingdom.
出版信息
Proc Natl Acad Sci U S A. 2011 Jul 12;108(28):11338-43. doi: 10.1073/pnas.1101519108. Epub 2011 Jun 27.
Abstract
A triazole mimic of a DNA phosphodiester linkage has been produced by templated chemical ligation of oligonucleotides functionalized with 5'-azide and 3'-alkyne. The individual azide and alkyne oligonucleotides were synthesized by standard phosphoramidite methods and assembled using a straightforward ligation procedure. This highly efficient chemical equivalent of enzymatic DNA ligation has been used to assemble a 300-mer from three 100-mer oligonucleotides, demonstrating the total chemical synthesis of very long oligonucleotides. The base sequences of the DNA strands containing this artificial linkage were copied during PCR with high fidelity and a gene containing the triazole linker was functional in Escherichia coli.
摘要
通过 5'-叠氮化物和 3'-炔烃官能化的寡核苷酸的模板化学连接，合成了 DNA 磷酸二酯键的三唑类似物。单个叠氮化物和炔烃寡核苷酸通过标准的亚磷酰胺方法合成，并使用简单的连接程序组装。这种酶促 DNA 连接的高效化学等价物已用于从三个 100 -mer 寡核苷酸组装 300-mer，证明了非常长的寡核苷酸的全化学合成。含有这种人工键的 DNA 链的碱基序列在 PCR 中具有高保真度进行复制，并且含有三唑接头的基因在大肠杆菌中具有功能。
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