Institute of Virology, University of Bonn Medical Centre, Sigmund Freud Str. 25, 53127 Bonn, Germany.
J Virol. 2011 Sep;85(17):9227-34. doi: 10.1128/JVI.00230-11. Epub 2011 Jun 29.
The family Bunyaviridae is the most diversified family of RNA viruses. We describe a novel prototypic bunyavirus, tentatively named Gouléako virus, isolated from various mosquito species trapped in Côte d'Ivoire. The S segment comprised 1,087 nucleotides (nt), the M segment 3,188 nt, and the L segment 6,358 nt, constituting the shortest bunyavirus genome known so far. The virus had shorter genome termini than phleboviruses and showed no evidence of encoded NSs and NSm proteins. An uncharacterized 105-amino-acid (aa) putative open reading frame (ORF) was detected in the S segment. Genetic equidistance to other bunyaviruses (74 to 88% aa identity) and absence of serological cross-reactivity with phleboviruses suggested a proposed novel Bunyaviridae genus.
布尼亚病毒科是 RNA 病毒中最多样化的家族。我们描述了一种新型的布尼亚病毒原型，暂命名为 Gouléako 病毒，该病毒从科特迪瓦捕获的各种蚊子中分离出来。S 片段由 1087 个核苷酸（nt）组成，M 片段由 3188 个 nt 组成，L 片段由 6358 个 nt 组成，构成了迄今已知的最短布尼亚病毒基因组。该病毒的基因组末端比黄病毒短，且没有编码 NSs 和 NSm 蛋白的证据。在 S 片段中检测到一个未表征的 105 个氨基酸（aa）的推定开放阅读框（ORF）。与其他布尼亚病毒的遗传等距（74%到 88%的 aa 同一性）和与黄病毒缺乏血清学交叉反应表明该病毒属于一个新提出的布尼亚病毒科属。
