Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, NY 10032, USA.
Virus Res. 2010 Jan;147(1):17-24. doi: 10.1016/j.virusres.2009.09.013. Epub 2009 Oct 3.
Characterization of arboviruses at the interface of pristine habitats and anthropogenic landscapes is crucial to comprehensive emergent disease surveillance and forecasting efforts. In context of a surveillance campaign in and around a West African rainforest, particles morphologically consistent with rhabdoviruses were identified in cell cultures infected with homogenates of trapped mosquitoes. RNA recovered from these cultures was used to derive the first complete genome sequence of a rhabdovirus isolated from Culex decens mosquitoes in Côte d'Ivoire, tentatively named Moussa virus (MOUV). MOUV shows the classical genome organization of rhabdoviruses, with five open reading frames (ORF) in a linear order. However, sequences show only limited conservation (12-33% identity at amino acid level), and ORF2 and ORF3 have no significant similarity to sequences deposited in GenBank. Phylogenetic analysis indicates a potential new species with distant relationship to Tupaia and Tibrogargan virus.
在原始生境和人为景观的交界处对虫媒病毒进行特征描述，对于全面的新发疾病监测和预测工作至关重要。在对西非雨林及其周边地区进行监测活动期间，从感染了捕获的蚊子匀浆的细胞培养物中鉴定出形态上与弹状病毒一致的颗粒。从这些培养物中回收的 RNA 用于推导从科特迪瓦的库蚊（Culex decens）中分离出的一株拉沙病毒（Moussa 病毒，MOUV）的首个完整基因组序列。MOUV 显示出弹状病毒的典型基因组组织，具有线性排列的五个开放阅读框（ORF）。然而，序列仅显示有限的保守性（氨基酸水平的 12-33%同一性），并且 ORF2 和 ORF3 与 GenBank 中存储的序列没有显著相似性。系统发育分析表明，它是与树鼩和提布罗加甘病毒具有遥远关系的潜在新种。
