Myers C R, Nealson K H
Center for Great Lakes Studies, University of Wisconsin-Milwaukee 53201.
J Bacteriol. 1990 Nov;172(11):6232-8. doi: 10.1128/jb.172.11.6232-6238.1990.
An oxidant pulse technique, with lactate as the electron donor, was used to study respiration-linked proton translocation in the manganese- and iron-reducing bacterium Shewanella putrefaciens MR-1. Cells grown anaerobically with fumarate or nitrate as the electron acceptor translocated protons in response to manganese (IV), fumarate, or oxygen. Cells grown anaerobically with fumarate also translocated protons in response to iron(III) and thiosulfate, whereas those grown with nitrate did not. Aerobically grown cells translocated protons only in response to oxygen. Proton translocation with all electron acceptors was abolished in the presence of the protonophore carbonyl cyanide m-chlorophenylhydrazone (20 microM) and was partially to completely inhibited by the electron transport inhibitor 2-n-heptyl-4-hydroxyquinoline N-oxide (50 microM).
采用以乳酸作为电子供体的氧化剂脉冲技术，研究了锰还原和铁还原细菌腐败希瓦氏菌MR-1中与呼吸相关的质子转运。以富马酸盐或硝酸盐作为电子受体进行厌氧培养的细胞，会因锰（IV）、富马酸盐或氧气而发生质子转运。以富马酸盐进行厌氧培养的细胞，也会因铁（III）和硫代硫酸盐而发生质子转运，而以硝酸盐进行厌氧培养的细胞则不会。需氧培养的细胞仅因氧气而发生质子转运。在质子载体羰基氰化物间氯苯腙（20微摩尔）存在的情况下，所有电子受体的质子转运均被消除，并且被电子传递抑制剂2-正庚基-4-羟基喹啉N-氧化物（50微摩尔）部分至完全抑制。
