二环己基碳二亚胺对大肠杆菌K-12厌氧生长细胞中与延胡索酸还原偶联的质子转运的影响。
Effects of dicyclohexylcarbodi-imide on proton translocation coupled to fumarate reduction in anaerobically grown cells of Escherichia coli K-12.
作者信息
Gutowski S J, Rosenberg H
出版信息
Biochem J. 1976 Dec 15;160(3):813-6. doi: 10.1042/bj1600813.
Abstract
The addition of dicyclohexylcarbodi-imide to anaerobic cells of Escherichia coli K12 decreases both the observed extent of proton translocation coupled to fumarate reduction by endogenous substrates and the t 1/2 of proton re-entry after such translocation, but does not affect fumarate uptake. Dicyclohexylcarbodi-imide also inhibits fumarate reductase activity in cell extracts.
摘要
向大肠杆菌K12的厌氧细胞中添加二环己基碳二亚胺，会降低与内源性底物将延胡索酸还原相偶联的质子转运的观察程度，以及这种转运后质子重新进入的半衰期，但不影响延胡索酸的摄取。二环己基碳二亚胺还会抑制细胞提取物中的延胡索酸还原酶活性。
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