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二环己基碳二亚胺对大肠杆菌K-12厌氧生长细胞中与延胡索酸还原偶联的质子转运的影响。

Effects of dicyclohexylcarbodi-imide on proton translocation coupled to fumarate reduction in anaerobically grown cells of Escherichia coli K-12.

作者信息

Gutowski S J, Rosenberg H

出版信息

Biochem J. 1976 Dec 15;160(3):813-6. doi: 10.1042/bj1600813.

DOI:10.1042/bj1600813
PMID:797390
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1164300/
Abstract

The addition of dicyclohexylcarbodi-imide to anaerobic cells of Escherichia coli K12 decreases both the observed extent of proton translocation coupled to fumarate reduction by endogenous substrates and the t 1/2 of proton re-entry after such translocation, but does not affect fumarate uptake. Dicyclohexylcarbodi-imide also inhibits fumarate reductase activity in cell extracts.

摘要

向大肠杆菌K12的厌氧细胞中添加二环己基碳二亚胺，会降低与内源性底物将延胡索酸还原相偶联的质子转运的观察程度，以及这种转运后质子重新进入的半衰期，但不影响延胡索酸的摄取。二环己基碳二亚胺还会抑制细胞提取物中的延胡索酸还原酶活性。

相似文献

1
Effects of dicyclohexylcarbodi-imide on proton translocation coupled to fumarate reduction in anaerobically grown cells of Escherichia coli K-12.二环己基碳二亚胺对大肠杆菌K-12厌氧生长细胞中与延胡索酸还原偶联的质子转运的影响。
Biochem J. 1976 Dec 15;160(3):813-6. doi: 10.1042/bj1600813.
2
Proton translocation coupled to electron flow from endogenous substrates to fumarate in anaerobically grown Escherichia coli K12.在厌氧生长的大肠杆菌K12中，质子转运与电子从内源性底物流向延胡索酸相偶联。
Biochem J. 1977 Apr 15;164(1):265-7. doi: 10.1042/bj1640265.
3
Proton translocation by the mitochondrial cytochrome b-c1 complex is inhibited by NN'-dicyclohexylcarbodi-imide.线粒体细胞色素b-c1复合物的质子转运受到N，N'-二环己基碳二亚胺的抑制。
Biochem J. 1982 Aug 15;206(2):419-21. doi: 10.1042/bj2060419.
4
Inhibition of the membrane-bound adenosine triphosphatase of Escherichia coli by dicyclohexylcarbodi-imide.二环己基碳二亚胺对大肠杆菌膜结合三磷酸腺苷酶的抑制作用。
Biochem J. 1977 Nov 1;167(2):497-9. doi: 10.1042/bj1670497.
5
[N,N'-dicyclohexylcarbodiimide-sensitive K+-dependent H+ secretion from anaerobically grown E. coli cells].[来自厌氧培养的大肠杆菌细胞的N，N'-二环己基碳二亚胺敏感的钾离子依赖性氢离子分泌]
Biofizika. 1989 Mar-Apr;34(2):322-3.
6
Thermodynamic and steady-state-kinetic investigation of the effect of NN'-dicyclohexylcarbodi-imide on H+ translocation by the mitochondrial cytochrome bc1 complex.N，N'-二环己基碳二亚胺对线粒体细胞色素bc1复合物质子转运影响的热力学和稳态动力学研究
Biochem J. 1985 Jan 15;225(2):407-11. doi: 10.1042/bj2250407.
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Sensitivity to NN'-dicyclohexylcarbodi-imide of proton translocation by mitochondrial NADH:ubiquinone oxidoreductase.线粒体NADH：泛醌氧化还原酶质子转运对N，N'-二环己基碳二亚胺的敏感性
Biochem J. 1986 Aug 1;237(3):927-30. doi: 10.1042/bj2370927.
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Functional anaerobic electron transport linked to the reduction of nitrate and fumarate in membranes from Escherichia coli as demonstrated by quenching of atebrin fluorescence.通过阿的平荧光猝灭证明，功能性厌氧电子传递与大肠杆菌膜中硝酸盐和富马酸盐的还原有关。
Biochem J. 1975 Dec;152(3):655-9. doi: 10.1042/bj1520655.
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Respiration-linked proton translocation coupled to anaerobic reduction of manganese(IV) and iron(III) in Shewanella putrefaciens MR-1.腐败希瓦氏菌MR-1中与锰（IV）和铁（III）的厌氧还原偶联的呼吸相关质子转运
J Bacteriol. 1990 Nov;172(11):6232-8. doi: 10.1128/jb.172.11.6232-6238.1990.
10
[N,N'-dicyclohexylcarbodiimide-sensitive K+-dependent ATPase activity in Escherichia coli].[大肠杆菌中对N,N'-二环己基碳二亚胺敏感的钾离子依赖性ATP酶活性]
Nauchnye Doki Vyss Shkoly Biol Nauki. 1989(5):90-5.

引用本文的文献

1
The respiratory chains of Escherichia coli.大肠杆菌的呼吸链
Microbiol Rev. 1984 Sep;48(3):222-71. doi: 10.1128/mr.48.3.222-271.1984.
2
Identification and localization of enzymes of the fumarate reductase and nitrate respiration systems of escherichia coli by crossed immunoelectrophoresis.通过交叉免疫电泳法对大肠杆菌延胡索酸还原酶和硝酸盐呼吸系统的酶进行鉴定和定位。
J Bacteriol. 1983 Feb;153(2):1027-37. doi: 10.1128/jb.153.2.1027-1037.1983.
3
Proton translocation coupled to electron flow from endogenous substrates to fumarate in anaerobically grown Escherichia coli K12.在厌氧生长的大肠杆菌K12中，质子转运与电子从内源性底物流向延胡索酸相偶联。
Biochem J. 1977 Apr 15;164(1):265-7. doi: 10.1042/bj1640265.

本文引用的文献

1
Membrane Mg-(Ca)-Activated Adenosine Triphosphatase of Escherichia coli: Characterization in the Membrane-Bound and Solubilized States.大肠杆菌膜镁（钙）激活的三磷酸腺苷酶：膜结合态和可溶态的特性研究
J Bacteriol. 1970 Dec;104(3):1203-12. doi: 10.1128/jb.104.3.1203-1212.1970.
2
The uptake of C4-dicarboxylic acids by Escherichia coli.大肠杆菌对C4-二羧酸的摄取
Eur J Biochem. 1971 Jan;18(2):274-81. doi: 10.1111/j.1432-1033.1971.tb01240.x.
3
Stoicheiometry of lactose-H+ symport across the plasma membrane of Escherichia coli.乳糖-H⁺同向转运体跨大肠杆菌质膜的化学计量学
Biochem J. 1973 Mar;132(3):587-92. doi: 10.1042/bj1320587.
4
Isolation and properties of fumarate reductase mutants of Escherichia coli.大肠杆菌延胡索酸还原酶突变体的分离与特性
J Bacteriol. 1973 May;114(2):563-70. doi: 10.1128/jb.114.2.563-570.1973.
5
A protonmotive force drives ATP synthesis in bacteria.质子动力驱使细菌中ATP的合成。
Proc Natl Acad Sci U S A. 1974 Oct;71(10):3896-900. doi: 10.1073/pnas.71.10.3896.
6
Effect of dicyclohexylcarbodiimide on growth and membrane-mediated processes in wild type and heptose-deficient mutants of Escherichia coli K-12.二环己基碳二亚胺对大肠杆菌K-12野生型和庚糖缺陷型突变体生长及膜介导过程的影响
J Bacteriol. 1974 Jul;119(1):129-37. doi: 10.1128/jb.119.1.129-137.1974.
7
Energization of active transport by Escherichia coli.大肠杆菌对主动运输的供能作用
J Biol Chem. 1972 Nov 25;247(22):7257-65.
8
Cation transport and electrogenesis by Streptococcus faecalis. II. Proton and sodium extrusion.粪链球菌的阳离子转运与电发生。II. 质子和钠的排出。
J Membr Biol. 1972;8(1):45-62. doi: 10.1007/BF01868094.
9
Oxidative phosphorylation in Escherichia coli K-12: the genetic and biochemical characterisations of a strain carrying a mutation in the uncB gene.大肠杆菌K-12中的氧化磷酸化：一株uncB基因发生突变的菌株的遗传与生化特性
Biochim Biophys Acta. 1973 Feb 22;292(2):366-75. doi: 10.1016/0005-2728(73)90043-1.
10
Energy coupling to active transport in anaerobically grown mutants of Escherichia Coli K12.大肠杆菌K12厌氧生长突变体中能量与主动运输的偶联
Biochem J. 1976 Mar 15;154(3):731-4. doi: 10.1042/bj1540731.