壁画分离菌的研究，导致“摩洛哥芽孢杆菌”和“胡萝卜芽孢杆菌”被归入简单芽孢杆菌，对简单芽孢杆菌的描述进行修订，重新检验先前归为“大芽孢杆菌”的菌株，并描述新种壁画芽孢杆菌
Study of mural painting isolates, leading to the transfer of 'Bacillus maroccanus' and 'Bacillus carotarum' to Bacillus simplex, emended description of Bacillus simplex, re-examination of the strains previously attributed to 'Bacillus macroides' and description of Bacillus muralis sp. nov.
作者信息
Heyrman Jeroen, Logan Niall A, Rodríguez-Díaz Marina, Scheldeman Patsy, Lebbe Liesbeth, Swings Jean, Heyndrickx Marc, De Vos Paul
机构信息
Vakgroep BFM WE10V, Laboratorium voor Microbiologie, Universiteit Gent, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium.
Department of Biological and Biomedical Sciences, Glasgow Caledonian University, Cowcaddens Road, Glasgow G4 0BA, UK.
出版信息
Int J Syst Evol Microbiol. 2005 Jan;55(Pt 1):119-131. doi: 10.1099/ijs.0.63221-0.
A group of 24 strains was isolated from deteriorated mural paintings situated in Spain (necropolis of Carmona) and Germany (church of Greene-Kreiensen). (GTG)5-PCR genomic fingerprinting was performed on these strains to assess their genomic variability and the strains were delineated into four groups. Representatives were studied by 16S rRNA gene sequencing and were found to be closely related to Bacillus simplex and the species 'Bacillus macroides' (strain NCIMB 8796) and 'Bacillus maroccanus' (names not validly published) according to a fasta search. The close similarity between B. simplex, 'B. macroides' NCIMB 8796, 'B. maroccanus' and the mural painting isolates was confirmed by additional (GTG)5-PCR, ARDRA, FAME and SDS-PAGE analyses. Furthermore, these techniques revealed that strains of 'Bacillus carotarum', another name that has not been validly published, also showed high similarity to this group of organisms. On the other hand, it was shown that the strains labelled 'B. macroides' in different collections do not all belong to the same species. Strain NCIMB 8796 can be allocated to B. simplex, while strain DSM 54 (=ATCC 12905) shares the highest 16S rRNA gene sequence similarity with Bacillus sphaericus and Bacillus fusiformis (both around 98.6 %). On the basis of further DNA-DNA hybridization data and the study of phenotypic characteristics, one group of five mural painting strains was attributed to a novel species in the genus Bacillus, for which the name Bacillus muralis sp. nov. is proposed. Finally, the remaining mural painting strains, one (LMG 18508=NCIMB 8796) of two strains belonging to 'B. macroides' and strains belonging to 'B. maroccanus' and 'B. carotarum' are allocated to the species B. simplex and an emended description of B. simplex is given.
从西班牙（卡尔莫纳墓地）和德国（格林 - 克雷森教堂）受损壁画中分离出一组24株菌株。对这些菌株进行了(GTG)5-PCR基因组指纹分析以评估其基因组变异性，这些菌株被分为四组。通过16S rRNA基因测序对代表菌株进行研究，根据fasta搜索发现它们与简单芽孢杆菌以及“巨大芽孢杆菌”（菌株NCIMB 8796）和“摩洛哥芽孢杆菌”（名称未有效发表）密切相关。通过额外的(GTG)5-PCR、ARDRA、FAME和SDS-PAGE分析证实了简单芽孢杆菌、“巨大芽孢杆菌”NCIMB 8796、“摩洛哥芽孢杆菌”与壁画分离株之间的密切相似性。此外，这些技术还表明，“胡萝卜芽孢杆菌”（另一个未有效发表的名称）的菌株也与这组生物体表现出高度相似性。另一方面，结果表明不同保藏库中标记为“巨大芽孢杆菌”的菌株并非都属于同一物种。菌株NCIMB 8796可归为简单芽孢杆菌，而菌株DSM 54（=ATCC 12905）与球形芽孢杆菌和梭形芽孢杆菌的16S rRNA基因序列相似度最高（均约为98.6%）。基于进一步的DNA-DNA杂交数据和表型特征研究，一组五株壁画菌株被归为芽孢杆菌属的一个新物种，为此提出了新名称壁芽孢杆菌（Bacillus muralis sp. nov.）。最后，其余的壁画菌株，即属于“巨大芽孢杆菌”的两株菌株中的一株（LMG 18508 = NCIMB 8796）以及属于“摩洛哥芽孢杆菌”和“胡萝卜芽孢杆菌”的菌株被归为简单芽孢杆菌，并给出了简单芽孢杆菌的修订描述。
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