Politi Flávio A S, de Mello João C P, Migliato Ketylin F, Nepomuceno Andréa L A, Moreira Raquel R D, Pietro Rosemeire C L R
Department of Drugs and Medicines, School of Pharmaceutical Sciences of Araraquara, UNESP-São Paulo State University, Rodovia Araraquara-Jaú, Km 01, 14801-902, CP 502 Araraquara (SP), Brazil; E-Mails:
Int J Mol Sci. 2011;12(4):2757-68. doi: 10.3390/ijms12042757. Epub 2011 Apr 21.
Endopleura uchi is a typical Amazonian tree and its bark is popularly employed in the preparation of teas against myomas, arthritis, influenza, diarrhea and cancer. In this study, the antioxidant activity, cytotoxicity and antimicrobial activity of five different extracts of the bark, selected by their total tannin content, were assessed. The potential antioxidant activity of the extracts was determined by 2.2-diphenyl-1-picrylhydrazyl radical scavenging assay and the values found were very similar among the extracts and to the standards antioxidants used in the tests. Cytotoxicity analysis in mammalian cells indicated that all the tested extracts exhibited IC(50) values higher than the highest concentration used, showing that they do not present a risk when consumed under these conditions. Extract tested against five bacterial strains and one yeast strain did not show satisfactory growth inhibitory activity, and even the extracts that showed some antimicrobial activity were not effective at any dilution to determine the minimum inhibitory concentration. The results may serve as a reference for subsequent works, since such reference values described in the literature for the bark of E. uchi.
内果皮树是一种典型的亚马逊树木，其树皮在民间常用于制备治疗肌瘤、关节炎、流感、腹泻和癌症的茶剂。在本研究中，对根据总单宁含量挑选出的该树皮的五种不同提取物的抗氧化活性、细胞毒性和抗菌活性进行了评估。通过2,2-二苯基-1-苦基肼自由基清除试验测定提取物的潜在抗氧化活性，发现各提取物的值与测试中使用的标准抗氧化剂非常相似。对哺乳动物细胞的细胞毒性分析表明，所有测试提取物的半数抑制浓度（IC50）值均高于所用的最高浓度，表明在这些条件下食用时它们不存在风险。针对五种细菌菌株和一种酵母菌株测试的提取物未显示出令人满意的生长抑制活性，即使显示出一定抗菌活性的提取物在任何稀释度下都无法有效确定最低抑菌浓度。这些结果可为后续研究提供参考，因为关于内果皮树树皮的此类参考值在文献中有记载。
