科罗拉多州库蚊携带的昆虫特异性黄病毒，有垂直传播的证据。
Insect-specific flaviviruses from Culex mosquitoes in Colorado, with evidence of vertical transmission.
机构信息
Arthropod-Borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado 80521, USA.
出版信息
Am J Trop Med Hyg. 2011 Jul;85(1):169-77. doi: 10.4269/ajtmh.2011.10-0474.
Mosquitoes were collected in Colorado during 2006 and 2007 to examine spatial and seasonal patterns of risk for exposure to Culex vectors and West Nile virus. We used universal flavivirus primers to test pools of Culex mosquitoes for viral RNA. This led to the detection and subsequent isolation of two insect-specific flaviviruses: Culex flavivirus (CxFV), which was first described from Japan, and a novel insect flavivirus, designated Calbertado virus (CLBOV), which has also been detected in California and Canada. We recorded both viruses in Cx. tarsalis and Cx. pipiens from Colorado. Furthermore, quantitative reverse transcription polymerase chain reaction (RT-PCR) revealed the presence of CxFV RNA in Cx. pipiens eggs and larvae from a laboratory colony established in 2005 and naturally infected with CxFV, suggesting vertical transmission as a means of viral maintenance in natural Culex populations. Finally, we present phylogenetic analyses of the relationships between insect-specific flaviviruses and other selected flaviviruses.
2006 年和 2007 年，我们在科罗拉多州收集了蚊子，以研究接触库蚊属媒介和西尼罗河病毒的风险的空间和季节性模式。我们使用通用黄病毒引物来测试库蚊蚊子的病毒 RNA 池。这导致了两种昆虫特异性黄病毒的检测和随后的分离：首次从日本描述的库蚊黄病毒（CxFV），以及一种新型昆虫黄病毒，命名为卡尔伯特多病毒（CLBOV），也在加利福尼亚和加拿大检测到。我们在科罗拉多州的库蚊属和库蚊属中都记录到了这两种病毒。此外，定量逆转录聚合酶链反应（RT-PCR）显示，在 2005 年建立的、自然感染 CxFV 的实验室群体中，库蚊属的卵和幼虫中存在 CxFV RNA，这表明垂直传播是在自然库蚊种群中维持病毒的一种手段。最后，我们提出了昆虫特异性黄病毒与其他选定黄病毒之间关系的系统发育分析。
Zotero 插件