Momou K J, Akoua-Koffi C, Akré D S, Adjogoua E V, Tiéoulou L, Dosso M
Unité des virus du système nerveux, département virus épidémique, institut Pasteur de Côte d'Ivoire, BP 490, Abidjan 01, Côte d'Ivoire.
Pathol Biol (Paris). 2012 Jun;60(3):e21-6. doi: 10.1016/j.patbio.2011.05.004. Epub 2011 Jul 7.
The aim of this study was to determine prevalence of enteroviruses in raw municipal wastewater and specify serotypes circulating.
After virus concentration by WHO recommend method, aqueous phase of 68 samples was inoculate on BGM, RD, L20B and Hep2 cells lines. Typing after titration was performed by microplate neutralization test with specific horse immune serum against EV (kit RIVM/WHO). NPEV strains were confirmed by RT-PCR test in 5'NC genomic region. Antigenic and molecular tests of Polioviruses intratypic differentiation were performed to determine vaccine or wild origin of Poliovirus strains.
Viral isolation obtained 43/68 (65.15 %) positives samples. Among these 35/43 (81. 39%) were EV and 8/43 (19.04 %) non EV strain. Among EVs, 34/35 (97.14 %) were NEPV and 2/35 (5.71 %) vaccinal Poliovirus strains type 2 (Sabin-like). NEPV typing gave 77 % untypeable strains by conventionally method. The average virus concentration estimated in the raw sewage is 4.28×10(5) PFU/L.
Various contacts between people and these waters expose them to health risks.
本研究旨在确定城市原污水中肠道病毒的流行情况，并明确其传播的血清型。
按照世界卫生组织推荐的方法进行病毒浓缩后，将68份样本的水相接种于BGM、RD、L20B和Hep2细胞系。滴定后通过使用针对肠道病毒的特异性马免疫血清（RIVM/世界卫生组织试剂盒）的微孔板中和试验进行分型。通过在5'NC基因组区域进行RT-PCR试验确认非脊髓灰质炎肠道病毒（NPEV）毒株。进行脊髓灰质炎病毒型内分化的抗原和分子试验，以确定脊髓灰质炎病毒毒株的疫苗或野生来源。
病毒分离获得43/68（65.15%）份阳性样本。其中，35/43（81.39%）为肠道病毒，8/43（19.04%）为非肠道病毒毒株。在肠道病毒中，34/35（97.14%）为NPEV，2/35（5.71%）为2型疫苗脊髓灰质炎病毒毒株（类似萨宾株）。通过传统方法，NPEV分型结果显示77%的毒株无法分型。原污水中估计的平均病毒浓度为4.28×10(5) PFU/L。
人与这些水体之间的各种接触使他们面临健康风险。
