Chagas Disease Multidisciplinary Research Laboratory, Faculty of Medicine, University of Brasília, Federal District, Brazil.
J Proteomics. 2011 Aug 24;74(9):1664-72. doi: 10.1016/j.jprot.2011.06.022. Epub 2011 Jul 1.
The triatomines in the tribe Rhodniini are the main vectors of the Trypanosoma cruzi to humans in recent outbreaks of acute Chagas disease in the Amazon. These insects dwelling in palm trees do not colonize the human domicile. Their success to transmit the infection relies partially on the efficacy of their salivary gland apparatuses. Here we show the transcriptome of the Rhodnius brethesi and Rhodnius robustus salivary glands, comprising 56 and 122 clusters, respectively. Approximately one third of these clusters are described for the first time. The LC-MS/MS analysis identified 123 and 111 proteins in R. brethesi and R. robustus sialome, respectively. Noteworthy, lipocalin platelet aggregation inhibitors, inositol polyphosphate 5-phosphatases, and Kazal domain proteins, which are essential for the insect's successful acquisition of blood meals, were found in our analysis. Moreover, glutathione S transferase and antigen-5, which play roles in the insect's defense and resistance against insecticide, were also observed.
在亚马逊地区最近爆发的急性克氏锥虫病中，Rhodniini 部落的三锥虫是克氏锥虫向人类传播的主要媒介。这些栖息在棕榈树上的昆虫不会在人类住所中筑巢。它们成功传播感染的部分原因在于其唾液腺器官的功效。在这里，我们展示了 Rhodnius brethesi 和 Rhodnius robustus 唾液腺的转录组，分别包含 56 和 122 个聚类。这些聚类中约有三分之一是首次描述的。LC-MS/MS 分析分别在 R. brethesi 和 R. robustus 的唾液中鉴定出 123 和 111 种蛋白质。值得注意的是，在我们的分析中发现了对昆虫成功获取血餐至关重要的脂联素血小板聚集抑制剂、肌醇多磷酸 5-磷酸酶和 Kazal 结构域蛋白。此外，还观察到谷胱甘肽 S 转移酶和抗原 5，它们在昆虫的防御和对杀虫剂的抗性中发挥作用。
